Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake
Background & aims: Exercise activates muscle pyruvate dehydrogenase complex (PDC), but moderate intensity exercise fails to fully activate muscle PDC after high-fat diet [1]. We investigated whether maximal intensity exercise overcomes this inhibition. Methods: Quadriceps femoris muscle biopsy...
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| Format: | Article |
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Elsevier
2018
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| Online Access: | https://eprints.nottingham.ac.uk/50275/ |
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| author | Constantin-Teodosiu, Dumitru Cederblad, Gitten Bergstrom, Mats Greenhaff, Paul L. |
| author_facet | Constantin-Teodosiu, Dumitru Cederblad, Gitten Bergstrom, Mats Greenhaff, Paul L. |
| author_sort | Constantin-Teodosiu, Dumitru |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background & aims: Exercise activates muscle pyruvate dehydrogenase complex (PDC), but moderate intensity exercise fails to fully activate muscle PDC after high-fat diet [1]. We investigated whether maximal intensity exercise overcomes this inhibition.
Methods: Quadriceps femoris muscle biopsy samples were obtained from healthy males at rest, and after 46 and 92 electrically-evoked maximal intermittent isometric contractions, which were preceded by 3 days of either low- (18%) or high- (69%) isocaloric dietary fat intake (LFD and HFD, respectively).
Results: The ratio of PDCa (active form) to total PDCt (fully activated) at rest was 50% less after HFD (0.32 ± 0.01 vs 0.15 ± 0.01; P<0.05). This ratio increased to 0.77 ± 0.06 after 46 contractions (P<0.001) and to 0.98 ± 0.07 after 92 contractions (P<0.001) in LFD. The corresponding values after HFD were less (0.54 ± 0.06; P<0.01 and 0.70 ± 0.07; P<0.01, respectively). Resting muscle acetyl-CoA and acetylcarnitine content was greater after HFD than LFD (both P<0.05), but their rate of accumulation in the former was reduced during contraction. Muscle lactate content after 92 contractions was 30% greater after HFD (P<0.05). Muscle force generation during contraction was no different between interventions, but HFD lengthened muscle relaxation time (P<0.05). Daily urinary total carnitine excretion after HFD was 2.5-fold greater than after LFD (P<0.01).
Conclusions: A bout of maximal intense exercise did not overcome dietary fat-mediated inhibition of muscle pyruvate dehydrogenase complex activation, and was associated with greater muscle lactate accumulation, as a result of lower PDC flux, and increased muscle relaxation time. |
| first_indexed | 2025-11-14T20:16:05Z |
| format | Article |
| id | nottingham-50275 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:16:05Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-502752024-08-15T15:26:39Z https://eprints.nottingham.ac.uk/50275/ Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake Constantin-Teodosiu, Dumitru Cederblad, Gitten Bergstrom, Mats Greenhaff, Paul L. Background & aims: Exercise activates muscle pyruvate dehydrogenase complex (PDC), but moderate intensity exercise fails to fully activate muscle PDC after high-fat diet [1]. We investigated whether maximal intensity exercise overcomes this inhibition. Methods: Quadriceps femoris muscle biopsy samples were obtained from healthy males at rest, and after 46 and 92 electrically-evoked maximal intermittent isometric contractions, which were preceded by 3 days of either low- (18%) or high- (69%) isocaloric dietary fat intake (LFD and HFD, respectively). Results: The ratio of PDCa (active form) to total PDCt (fully activated) at rest was 50% less after HFD (0.32 ± 0.01 vs 0.15 ± 0.01; P<0.05). This ratio increased to 0.77 ± 0.06 after 46 contractions (P<0.001) and to 0.98 ± 0.07 after 92 contractions (P<0.001) in LFD. The corresponding values after HFD were less (0.54 ± 0.06; P<0.01 and 0.70 ± 0.07; P<0.01, respectively). Resting muscle acetyl-CoA and acetylcarnitine content was greater after HFD than LFD (both P<0.05), but their rate of accumulation in the former was reduced during contraction. Muscle lactate content after 92 contractions was 30% greater after HFD (P<0.05). Muscle force generation during contraction was no different between interventions, but HFD lengthened muscle relaxation time (P<0.05). Daily urinary total carnitine excretion after HFD was 2.5-fold greater than after LFD (P<0.01). Conclusions: A bout of maximal intense exercise did not overcome dietary fat-mediated inhibition of muscle pyruvate dehydrogenase complex activation, and was associated with greater muscle lactate accumulation, as a result of lower PDC flux, and increased muscle relaxation time. Elsevier 2018-02-15 Article PeerReviewed Constantin-Teodosiu, Dumitru, Cederblad, Gitten, Bergstrom, Mats and Greenhaff, Paul L. (2018) Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake. Clinical Nutrition . ISSN 1532-1983 Diet ; Maximal intensity contraction ; PDC activation ; Carnitine https://www.sciencedirect.com/science/article/pii/S0261561418300645 doi:10.1016/j.clnu.2018.02.001 doi:10.1016/j.clnu.2018.02.001 |
| spellingShingle | Diet ; Maximal intensity contraction ; PDC activation ; Carnitine Constantin-Teodosiu, Dumitru Cederblad, Gitten Bergstrom, Mats Greenhaff, Paul L. Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title | Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title_full | Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title_fullStr | Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title_full_unstemmed | Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title_short | Maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| title_sort | maximal-intensity exercise does not fully restore muscle pyruvate dehydrogenase complex activation after 3 days of high-fat dietary intake |
| topic | Diet ; Maximal intensity contraction ; PDC activation ; Carnitine |
| url | https://eprints.nottingham.ac.uk/50275/ https://eprints.nottingham.ac.uk/50275/ https://eprints.nottingham.ac.uk/50275/ |