B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass

Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species – including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass (Lolium perenne L.), the presence of B chromosomes...

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Main Authors: Harper, John, Phillips, Dylan, Thomas, Ann, Gasior, Dagmara, Evans, Caron, Powell, Wayne, King, Julie, King, Ian, Jenkins, Glyn, Armstead, Ian
Format: Article
Language:English
English
Published: Oxford University Press 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/50056/
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author Harper, John
Phillips, Dylan
Thomas, Ann
Gasior, Dagmara
Evans, Caron
Powell, Wayne
King, Julie
King, Ian
Jenkins, Glyn
Armstead, Ian
author_facet Harper, John
Phillips, Dylan
Thomas, Ann
Gasior, Dagmara
Evans, Caron
Powell, Wayne
King, Julie
King, Ian
Jenkins, Glyn
Armstead, Ian
author_sort Harper, John
building Nottingham Research Data Repository
collection Online Access
description Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species – including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass (Lolium perenne L.), the presence of B chromosomes has been reported as influencing both chromosome pairing and chiasma frequencies. In this study, the effects of the presence/absence of B chromosomes on genetic recombination has been investigated through generating DArT marker genetic maps for 6 perennial ryegrass diploid populations, the pollen parents of which contained either two B or zero B chromosomes. Through genetic and cytological analyses of these progenies and their parents we have identified that, while overall cytological estimates of chiasma frequencies were significantly lower in pollen mother cells with two B chromosomes as compared to zero B chromosomes, the recombination frequencies within some marker intervals were actually increased, particularly for marker intervals in lower recombination regions of chromosomes, i.e., pericentromeric regions. Thus, in perennial ryegrass, the presence of two B chromosomes redistributed patterns of meiotic recombination in pollen mother cells in ways which could increase the range of allelic variation available to plant breeders.
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spelling nottingham-500562020-05-08T09:01:51Z https://eprints.nottingham.ac.uk/50056/ B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass Harper, John Phillips, Dylan Thomas, Ann Gasior, Dagmara Evans, Caron Powell, Wayne King, Julie King, Ian Jenkins, Glyn Armstead, Ian Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species – including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass (Lolium perenne L.), the presence of B chromosomes has been reported as influencing both chromosome pairing and chiasma frequencies. In this study, the effects of the presence/absence of B chromosomes on genetic recombination has been investigated through generating DArT marker genetic maps for 6 perennial ryegrass diploid populations, the pollen parents of which contained either two B or zero B chromosomes. Through genetic and cytological analyses of these progenies and their parents we have identified that, while overall cytological estimates of chiasma frequencies were significantly lower in pollen mother cells with two B chromosomes as compared to zero B chromosomes, the recombination frequencies within some marker intervals were actually increased, particularly for marker intervals in lower recombination regions of chromosomes, i.e., pericentromeric regions. Thus, in perennial ryegrass, the presence of two B chromosomes redistributed patterns of meiotic recombination in pollen mother cells in ways which could increase the range of allelic variation available to plant breeders. Oxford University Press 2018-04-09 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/50056/8/ery052.pdf application/pdf en https://eprints.nottingham.ac.uk/50056/1/King_et_al-2017-Plant_Biotechnology_Journal.pdf Harper, John, Phillips, Dylan, Thomas, Ann, Gasior, Dagmara, Evans, Caron, Powell, Wayne, King, Julie, King, Ian, Jenkins, Glyn and Armstead, Ian (2018) B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass. Journal of Experimental Botany, 69 (8). pp. 1861-1871. ISSN 1460-2431 B chromosomes chiasma genetic mapping Lolium perenne meiosis perennial ryegrass recombination https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/ery052/4948791 doi:10.1093/jxb/ery052 doi:10.1093/jxb/ery052
spellingShingle B chromosomes
chiasma
genetic mapping
Lolium perenne
meiosis
perennial ryegrass
recombination
Harper, John
Phillips, Dylan
Thomas, Ann
Gasior, Dagmara
Evans, Caron
Powell, Wayne
King, Julie
King, Ian
Jenkins, Glyn
Armstead, Ian
B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title_full B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title_fullStr B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title_full_unstemmed B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title_short B chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
title_sort b chromosomes are associated with redistribution of genetic recombination towards lower-recombination chromosomal regions in perennial ryegrass
topic B chromosomes
chiasma
genetic mapping
Lolium perenne
meiosis
perennial ryegrass
recombination
url https://eprints.nottingham.ac.uk/50056/
https://eprints.nottingham.ac.uk/50056/
https://eprints.nottingham.ac.uk/50056/