2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions
Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha‐proteobacterium Sinorhizobium meliloti, the lack of long‐chain fatty acyl‐coenzyme A synthetase activity (FadD) leads to in...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018
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| Online Access: | https://eprints.nottingham.ac.uk/49996/ |
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| author | López-Lara, Isabel M. Nogales, Joaquina Pech-Canul, Angel Calatrava-Morales, Nieves Bernabéu-Roda, Lydia M. Durán, Paoloma Cuéllar, Virginia Olivares, José Alvarez, Laura Palenzuela-Bretones, Diana Romero, Manuel Heeb, Stephan Cámara, Miguel Geiger, Otto Soto, Maria J. |
| author_facet | López-Lara, Isabel M. Nogales, Joaquina Pech-Canul, Angel Calatrava-Morales, Nieves Bernabéu-Roda, Lydia M. Durán, Paoloma Cuéllar, Virginia Olivares, José Alvarez, Laura Palenzuela-Bretones, Diana Romero, Manuel Heeb, Stephan Cámara, Miguel Geiger, Otto Soto, Maria J. |
| author_sort | López-Lara, Isabel M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha‐proteobacterium Sinorhizobium meliloti, the lack of long‐chain fatty acyl‐coenzyme A synthetase activity (FadD) leads to increased surface motility, defects in biofilm development, and impaired root colonization. In this study, analyses of lipid extracts and volatiles revealed that a fadD mutant accumulates 2‐tridecanone (2‐TDC), a methylketone known as a natural insecticide. Application of pure 2‐TDC to the wild‐type strain phenocopies the free‐living and symbiotic behaviours of the fadD mutant. Structural features of the methylketone determine its ability to promote S. meliloti surface translocation, which is mainly mediated by a flagella‐independent motility. Transcriptomic analyses showed that 2‐TDC induces differential expression of iron uptake, redox, and stress‐related genes. Interestingly, this methylketone also influences surface motility and impairs biofilm formation in plant and animal pathogenic bacteria. Moreover, 2‐TDC not only hampers alfalfa nodulation but also the development of tomato bacterial speck disease. This work assigns a new role to 2‐TDC as an infochemical that affects important bacterial traits and hampers plant‐bacteria interactions by interfering with microbial colonization of plant tissues. |
| first_indexed | 2025-11-14T20:14:48Z |
| format | Article |
| id | nottingham-49996 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:14:48Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-499962019-02-28T04:30:18Z https://eprints.nottingham.ac.uk/49996/ 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions López-Lara, Isabel M. Nogales, Joaquina Pech-Canul, Angel Calatrava-Morales, Nieves Bernabéu-Roda, Lydia M. Durán, Paoloma Cuéllar, Virginia Olivares, José Alvarez, Laura Palenzuela-Bretones, Diana Romero, Manuel Heeb, Stephan Cámara, Miguel Geiger, Otto Soto, Maria J. Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha‐proteobacterium Sinorhizobium meliloti, the lack of long‐chain fatty acyl‐coenzyme A synthetase activity (FadD) leads to increased surface motility, defects in biofilm development, and impaired root colonization. In this study, analyses of lipid extracts and volatiles revealed that a fadD mutant accumulates 2‐tridecanone (2‐TDC), a methylketone known as a natural insecticide. Application of pure 2‐TDC to the wild‐type strain phenocopies the free‐living and symbiotic behaviours of the fadD mutant. Structural features of the methylketone determine its ability to promote S. meliloti surface translocation, which is mainly mediated by a flagella‐independent motility. Transcriptomic analyses showed that 2‐TDC induces differential expression of iron uptake, redox, and stress‐related genes. Interestingly, this methylketone also influences surface motility and impairs biofilm formation in plant and animal pathogenic bacteria. Moreover, 2‐TDC not only hampers alfalfa nodulation but also the development of tomato bacterial speck disease. This work assigns a new role to 2‐TDC as an infochemical that affects important bacterial traits and hampers plant‐bacteria interactions by interfering with microbial colonization of plant tissues. Wiley 2018-02-28 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/49996/1/LopezLaraet%20al%20EnvironMicrobiol23.2.2018Proof1.pdf López-Lara, Isabel M., Nogales, Joaquina, Pech-Canul, Angel, Calatrava-Morales, Nieves, Bernabéu-Roda, Lydia M., Durán, Paoloma, Cuéllar, Virginia, Olivares, José, Alvarez, Laura, Palenzuela-Bretones, Diana, Romero, Manuel, Heeb, Stephan, Cámara, Miguel, Geiger, Otto and Soto, Maria J. (2018) 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions. Environmental Microbiology, 20 (6). pp. 2049-2065. ISSN 1462-2920 https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14083 doi:10.1111/1462-2920.14083 doi:10.1111/1462-2920.14083 |
| spellingShingle | López-Lara, Isabel M. Nogales, Joaquina Pech-Canul, Angel Calatrava-Morales, Nieves Bernabéu-Roda, Lydia M. Durán, Paoloma Cuéllar, Virginia Olivares, José Alvarez, Laura Palenzuela-Bretones, Diana Romero, Manuel Heeb, Stephan Cámara, Miguel Geiger, Otto Soto, Maria J. 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title | 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title_full | 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title_fullStr | 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title_full_unstemmed | 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title_short | 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| title_sort | 2-tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions |
| url | https://eprints.nottingham.ac.uk/49996/ https://eprints.nottingham.ac.uk/49996/ https://eprints.nottingham.ac.uk/49996/ |