Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity
Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing c...
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| Format: | Article |
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Elsevier
2015
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| Online Access: | https://eprints.nottingham.ac.uk/49964/ |
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| author | Marti, E. Wang, X. Jambari, N.N. Rhyner, C. Olzhausen, J. Pérez-Barea, J.J. Figueredo, Grazziela P. Alcocer, Marcos J.C. |
| author_facet | Marti, E. Wang, X. Jambari, N.N. Rhyner, C. Olzhausen, J. Pérez-Barea, J.J. Figueredo, Grazziela P. Alcocer, Marcos J.C. |
| author_sort | Marti, E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known. |
| first_indexed | 2025-11-14T20:14:44Z |
| format | Article |
| id | nottingham-49964 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:14:44Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-499642020-05-04T17:19:30Z https://eprints.nottingham.ac.uk/49964/ Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity Marti, E. Wang, X. Jambari, N.N. Rhyner, C. Olzhausen, J. Pérez-Barea, J.J. Figueredo, Grazziela P. Alcocer, Marcos J.C. Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known. Elsevier 2015-10-15 Article PeerReviewed Marti, E., Wang, X., Jambari, N.N., Rhyner, C., Olzhausen, J., Pérez-Barea, J.J., Figueredo, Grazziela P. and Alcocer, Marcos J.C. (2015) Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity. Veterinary Immunology and Immunopathology, 167 (3-4). pp. 171-177. ISSN 1873-2534 Protein array; Allergens; Chemometrics; IgE; Horse https://www.sciencedirect.com/science/article/pii/S0165242715001397?via%3Dihub doi:10.1016/j.vetimm.2015.06.013 doi:10.1016/j.vetimm.2015.06.013 |
| spellingShingle | Protein array; Allergens; Chemometrics; IgE; Horse Marti, E. Wang, X. Jambari, N.N. Rhyner, C. Olzhausen, J. Pérez-Barea, J.J. Figueredo, Grazziela P. Alcocer, Marcos J.C. Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title | Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title_full | Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title_fullStr | Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title_full_unstemmed | Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title_short | Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| title_sort | novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity |
| topic | Protein array; Allergens; Chemometrics; IgE; Horse |
| url | https://eprints.nottingham.ac.uk/49964/ https://eprints.nottingham.ac.uk/49964/ https://eprints.nottingham.ac.uk/49964/ |