Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin
Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Karger Publishers
2011
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| Online Access: | https://eprints.nottingham.ac.uk/49919/ |
| _version_ | 1848798110729371648 |
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| author | Rashidi, Hassan Strohbuecker, Stephanie Jackson, Leigh Kalra, Spandan Blake, Alexander J. France, Louise Tufarelli, Cristina Sottile, Virginie |
| author_facet | Rashidi, Hassan Strohbuecker, Stephanie Jackson, Leigh Kalra, Spandan Blake, Alexander J. France, Louise Tufarelli, Cristina Sottile, Virginie |
| author_sort | Rashidi, Hassan |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. |
| first_indexed | 2025-11-14T20:14:33Z |
| format | Article |
| id | nottingham-49919 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:14:33Z |
| publishDate | 2011 |
| publisher | Karger Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-499192018-12-04T17:29:14Z https://eprints.nottingham.ac.uk/49919/ Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin Rashidi, Hassan Strohbuecker, Stephanie Jackson, Leigh Kalra, Spandan Blake, Alexander J. France, Louise Tufarelli, Cristina Sottile, Virginie Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. Karger Publishers 2011-11-23 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/49919/1/Abstract/329861 Rashidi, Hassan, Strohbuecker, Stephanie, Jackson, Leigh, Kalra, Spandan, Blake, Alexander J., France, Louise, Tufarelli, Cristina and Sottile, Virginie (2011) Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin. Cells Tissues Organs, 195 (6). pp. 484-94. ISSN 1422-6421 https://www.karger.com/Article/Abstract/329861 doi:10.1159/000329861 doi:10.1159/000329861 |
| spellingShingle | Rashidi, Hassan Strohbuecker, Stephanie Jackson, Leigh Kalra, Spandan Blake, Alexander J. France, Louise Tufarelli, Cristina Sottile, Virginie Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title | Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title_full | Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title_fullStr | Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title_full_unstemmed | Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title_short | Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| title_sort | differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin |
| url | https://eprints.nottingham.ac.uk/49919/ https://eprints.nottingham.ac.uk/49919/ https://eprints.nottingham.ac.uk/49919/ |