SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade

Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stim...

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Main Authors: Xue, Wei, Metheringham, Rachael L., Brentville, Victoria A., Gunn, Barbara, Symonds, Peter, Yagita, Hideo, Ramage, Judith M., Durrant, Lindy
Format: Article
Published: Taylor & Francis 2016
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Online Access:https://eprints.nottingham.ac.uk/49788/
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author Xue, Wei
Metheringham, Rachael L.
Brentville, Victoria A.
Gunn, Barbara
Symonds, Peter
Yagita, Hideo
Ramage, Judith M.
Durrant, Lindy
author_facet Xue, Wei
Metheringham, Rachael L.
Brentville, Victoria A.
Gunn, Barbara
Symonds, Peter
Yagita, Hideo
Ramage, Judith M.
Durrant, Lindy
author_sort Xue, Wei
building Nottingham Research Data Repository
collection Online Access
description Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses.
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spelling nottingham-497882020-05-04T17:56:37Z https://eprints.nottingham.ac.uk/49788/ SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade Xue, Wei Metheringham, Rachael L. Brentville, Victoria A. Gunn, Barbara Symonds, Peter Yagita, Hideo Ramage, Judith M. Durrant, Lindy Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses. Taylor & Francis 2016-06-15 Article PeerReviewed Xue, Wei, Metheringham, Rachael L., Brentville, Victoria A., Gunn, Barbara, Symonds, Peter, Yagita, Hideo, Ramage, Judith M. and Durrant, Lindy (2016) SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade. Oncoimmunology, 5 (6). e1169353/1-e1169353/13. ISSN 2162-402X Cancer immunotherapy; CD4C T cells; CD8C T cells; targeting antigen-presenting cells; NY-ESO-1 https://www.ncbi.nlm.nih.gov/pubmed/27471648 doi:10.1080/2162402X.2016.1169353 doi:10.1080/2162402X.2016.1169353
spellingShingle Cancer immunotherapy; CD4C T cells; CD8C T cells; targeting antigen-presenting cells; NY-ESO-1
Xue, Wei
Metheringham, Rachael L.
Brentville, Victoria A.
Gunn, Barbara
Symonds, Peter
Yagita, Hideo
Ramage, Judith M.
Durrant, Lindy
SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title_full SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title_fullStr SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title_full_unstemmed SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title_short SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
title_sort scib2, an antibody dna vaccine encoding ny-eso-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade
topic Cancer immunotherapy; CD4C T cells; CD8C T cells; targeting antigen-presenting cells; NY-ESO-1
url https://eprints.nottingham.ac.uk/49788/
https://eprints.nottingham.ac.uk/49788/
https://eprints.nottingham.ac.uk/49788/