Identification of stable reference genes for quantitative PCR in koalas
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation. This study describes the identification and testing of reference genes with stable expression profiles in koala lymph node t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2018
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| Online Access: | https://eprints.nottingham.ac.uk/49705/ |
| _version_ | 1848798059127898112 |
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| author | Sarker, N. Fabijan, J. Emes, Richard D. Hemmatzadeh, F. Meers, J. Moreton, Joanna Owen, H. Seddon, J. Simmons, G. Speight, N. Trott, D. Woolford, L. Tarlinton, Rachael E. |
| author_facet | Sarker, N. Fabijan, J. Emes, Richard D. Hemmatzadeh, F. Meers, J. Moreton, Joanna Owen, H. Seddon, J. Simmons, G. Speight, N. Trott, D. Woolford, L. Tarlinton, Rachael E. |
| author_sort | Sarker, N. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation. This study describes the identification and testing of reference genes with stable expression profiles in koala lymph node tissues across two genetically distinct koala populations. From the 25 most stable genes identified in transcriptome analysis, 11 genes were selected for verification using reverse transcription quantitative PCR, in addition to the commonly used ACTB and GAPDH genes. The expression data were analysed using stable genes statistical software - geNorm, BestKeeper, NormFinder, the comparative ΔCt method and RefFinder. All 13 genes showed relative stability in expression in koala lymph node tissues, however Tmem97 and Hmg20a were identified as the most stable genes across the two koala populations. |
| first_indexed | 2025-11-14T20:13:45Z |
| format | Article |
| id | nottingham-49705 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:13:45Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-497052020-05-04T19:33:03Z https://eprints.nottingham.ac.uk/49705/ Identification of stable reference genes for quantitative PCR in koalas Sarker, N. Fabijan, J. Emes, Richard D. Hemmatzadeh, F. Meers, J. Moreton, Joanna Owen, H. Seddon, J. Simmons, G. Speight, N. Trott, D. Woolford, L. Tarlinton, Rachael E. To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation. This study describes the identification and testing of reference genes with stable expression profiles in koala lymph node tissues across two genetically distinct koala populations. From the 25 most stable genes identified in transcriptome analysis, 11 genes were selected for verification using reverse transcription quantitative PCR, in addition to the commonly used ACTB and GAPDH genes. The expression data were analysed using stable genes statistical software - geNorm, BestKeeper, NormFinder, the comparative ΔCt method and RefFinder. All 13 genes showed relative stability in expression in koala lymph node tissues, however Tmem97 and Hmg20a were identified as the most stable genes across the two koala populations. Nature Publishing Group 2018-02-20 Article PeerReviewed Sarker, N., Fabijan, J., Emes, Richard D., Hemmatzadeh, F., Meers, J., Moreton, Joanna, Owen, H., Seddon, J., Simmons, G., Speight, N., Trott, D., Woolford, L. and Tarlinton, Rachael E. (2018) Identification of stable reference genes for quantitative PCR in koalas. Scientific Reports, 8 . 3364/1-3364/8. ISSN 2045-2322 stable gene koala RT-qPCR https://www.nature.com/articles/s41598-018-21723-0 doi:10.1038/s41598-018-21723-0 doi:10.1038/s41598-018-21723-0 |
| spellingShingle | stable gene koala RT-qPCR Sarker, N. Fabijan, J. Emes, Richard D. Hemmatzadeh, F. Meers, J. Moreton, Joanna Owen, H. Seddon, J. Simmons, G. Speight, N. Trott, D. Woolford, L. Tarlinton, Rachael E. Identification of stable reference genes for quantitative PCR in koalas |
| title | Identification of stable reference genes for quantitative PCR in koalas |
| title_full | Identification of stable reference genes for quantitative PCR in koalas |
| title_fullStr | Identification of stable reference genes for quantitative PCR in koalas |
| title_full_unstemmed | Identification of stable reference genes for quantitative PCR in koalas |
| title_short | Identification of stable reference genes for quantitative PCR in koalas |
| title_sort | identification of stable reference genes for quantitative pcr in koalas |
| topic | stable gene koala RT-qPCR |
| url | https://eprints.nottingham.ac.uk/49705/ https://eprints.nottingham.ac.uk/49705/ https://eprints.nottingham.ac.uk/49705/ |