Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography

Bioluminescence imaging has shown great potential for studying and monitoring disease progression in small animal pre-clinical imaging. However, absolute bioluminescence source recovery through tomographic multi-wavelength measurements is often hindered through the lack of quantitative accuracy and...

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Main Authors: Jayet, Baptiste, Morgan, Stephen P., Dehgani, Hamid
Format: Article
Language:English
English
Published: Optical Society of America 2018
Online Access:https://eprints.nottingham.ac.uk/49666/
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author Jayet, Baptiste
Morgan, Stephen P.
Dehgani, Hamid
author_facet Jayet, Baptiste
Morgan, Stephen P.
Dehgani, Hamid
author_sort Jayet, Baptiste
building Nottingham Research Data Repository
collection Online Access
description Bioluminescence imaging has shown great potential for studying and monitoring disease progression in small animal pre-clinical imaging. However, absolute bioluminescence source recovery through tomographic multi-wavelength measurements is often hindered through the lack of quantitative accuracy and suffers from both poor localisation and quantitative recovery. In this work a method to incorporate a permissible region strategy through not only a priori location (permissible region) but also based on a model of light propagation and hence light sensitivity is developed and tested using both simulations and experimental data. Reconstructions on two different numerical models (a simple slab, and the digital version of a heterogeneous mouse) show an improvement of localisation and recovery of intensity (around 25% for the slab model and around 10% for the digital mouse model). This strategy is also used with experimental data from a phantom gel, which demonstrated an improved recovered tomographic image.
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spelling nottingham-496662020-05-08T12:00:31Z https://eprints.nottingham.ac.uk/49666/ Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography Jayet, Baptiste Morgan, Stephen P. Dehgani, Hamid Bioluminescence imaging has shown great potential for studying and monitoring disease progression in small animal pre-clinical imaging. However, absolute bioluminescence source recovery through tomographic multi-wavelength measurements is often hindered through the lack of quantitative accuracy and suffers from both poor localisation and quantitative recovery. In this work a method to incorporate a permissible region strategy through not only a priori location (permissible region) but also based on a model of light propagation and hence light sensitivity is developed and tested using both simulations and experimental data. Reconstructions on two different numerical models (a simple slab, and the digital version of a heterogeneous mouse) show an improvement of localisation and recovery of intensity (around 25% for the slab model and around 10% for the digital mouse model). This strategy is also used with experimental data from a phantom gel, which demonstrated an improved recovered tomographic image. Optical Society of America 2018-02-27 Article PeerReviewed application/pdf en cc_by https://eprints.nottingham.ac.uk/49666/8/boe-9-3-1360.pdf application/pdf en cc_by https://eprints.nottingham.ac.uk/49666/16/boe-9-3-1360.pdf Jayet, Baptiste, Morgan, Stephen P. and Dehgani, Hamid (2018) Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography. Biomedical Optics Express, 9 (3). pp. 1360-1374. ISSN 2156-7085 https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-3-1360 doi:10.1364/BOE.9.001360 doi:10.1364/BOE.9.001360
spellingShingle Jayet, Baptiste
Morgan, Stephen P.
Dehgani, Hamid
Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title_full Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title_fullStr Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title_full_unstemmed Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title_short Incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
title_sort incorporation of an ultrasound and model guided permissible region improves quantitative source recovery in bioluminescence tomography
url https://eprints.nottingham.ac.uk/49666/
https://eprints.nottingham.ac.uk/49666/
https://eprints.nottingham.ac.uk/49666/