Extraction of synaptic input properties in vivo
Knowledge of synaptic input is crucial for understanding synaptic integration and ultimately neural function. However, in vivo, the rates at which synaptic inputs arrive are high, so that it is typically impossible to detect single events. We show here that it is nevertheless possible to extract the...
| Main Authors: | , , , |
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| Format: | Article |
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MIT Press
2017
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| Online Access: | https://eprints.nottingham.ac.uk/49602/ |
| _version_ | 1848798035724730368 |
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| author | Puggioni, Paolo Jelitai, Marta Duguid, Ian van Rossum, Mark C.W. |
| author_facet | Puggioni, Paolo Jelitai, Marta Duguid, Ian van Rossum, Mark C.W. |
| author_sort | Puggioni, Paolo |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Knowledge of synaptic input is crucial for understanding synaptic integration and ultimately neural function. However, in vivo, the rates at which synaptic inputs arrive are high, so that it is typically impossible to detect single events. We show here that it is nevertheless possible to extract the properties of the events and, in particular, to extract the event rate, the synaptic time constants, and the properties of the event size distribution from in vivo voltage-clamp recordings. Applied to cerebellar interneurons, our method reveals that the synaptic input rate increases from 600 Hz during rest to 1000 Hz during locomotion, while the amplitude and shape of the synaptic events are unaffected by this state change. This method thus complements existing methods to measure neural function in vivo. |
| first_indexed | 2025-11-14T20:13:22Z |
| format | Article |
| id | nottingham-49602 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:13:22Z |
| publishDate | 2017 |
| publisher | MIT Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-496022020-05-04T19:56:43Z https://eprints.nottingham.ac.uk/49602/ Extraction of synaptic input properties in vivo Puggioni, Paolo Jelitai, Marta Duguid, Ian van Rossum, Mark C.W. Knowledge of synaptic input is crucial for understanding synaptic integration and ultimately neural function. However, in vivo, the rates at which synaptic inputs arrive are high, so that it is typically impossible to detect single events. We show here that it is nevertheless possible to extract the properties of the events and, in particular, to extract the event rate, the synaptic time constants, and the properties of the event size distribution from in vivo voltage-clamp recordings. Applied to cerebellar interneurons, our method reveals that the synaptic input rate increases from 600 Hz during rest to 1000 Hz during locomotion, while the amplitude and shape of the synaptic events are unaffected by this state change. This method thus complements existing methods to measure neural function in vivo. MIT Press 2017-07 Article PeerReviewed Puggioni, Paolo, Jelitai, Marta, Duguid, Ian and van Rossum, Mark C.W. (2017) Extraction of synaptic input properties in vivo. Neural Computation, 29 (7). pp. 1745-1768. ISSN 1530-888X https://www.mitpressjournals.org/doi/abs/10.1162/NECO_a_00975 doi:10.1162/NECO_a_00975 doi:10.1162/NECO_a_00975 |
| spellingShingle | Puggioni, Paolo Jelitai, Marta Duguid, Ian van Rossum, Mark C.W. Extraction of synaptic input properties in vivo |
| title | Extraction of synaptic input properties in vivo |
| title_full | Extraction of synaptic input properties in vivo |
| title_fullStr | Extraction of synaptic input properties in vivo |
| title_full_unstemmed | Extraction of synaptic input properties in vivo |
| title_short | Extraction of synaptic input properties in vivo |
| title_sort | extraction of synaptic input properties in vivo |
| url | https://eprints.nottingham.ac.uk/49602/ https://eprints.nottingham.ac.uk/49602/ https://eprints.nottingham.ac.uk/49602/ |