Highly anisotropic suspended planar-array chips with multidimensional sub-micrometric biomolecular patterns
Suspended planar-array (SPA) chips embody millions of individual miniaturized arrays to work in extremely small volumes. Here, the basis of a robust methodology for the fabrication of SPA silicon chips with on-demand physical and chemical anisotropies is demonstrated. Specifically, physical traits a...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/49510/ |
| _version_ | 1848798014706024448 |
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| author | Agusil, Juan Pablo Torras, Núria Duch, Marta Esteve, Jaume Pérez-García, Lluïsa Samitier, Josep Plaza, José A. |
| author_facet | Agusil, Juan Pablo Torras, Núria Duch, Marta Esteve, Jaume Pérez-García, Lluïsa Samitier, Josep Plaza, José A. |
| author_sort | Agusil, Juan Pablo |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Suspended planar-array (SPA) chips embody millions of individual miniaturized arrays to work in extremely small volumes. Here, the basis of a robust methodology for the fabrication of SPA silicon chips with on-demand physical and chemical anisotropies is demonstrated. Specifically, physical traits are defined during the fabrication process with special focus on the aspect ratio, branching, faceting, and size gradient of the final chips. Additionally, the chemical attributes augment the functionality of the chips with the inclusion of complete coverage or patterns of selected biomolecules on the surface of the chips with contact printing techniques, offering an extremely high versatility, not only with the choice of the pattern shape and distribution but also in the choice of biomolecular inks to pattern. This approach increases the miniaturization of printed arrays in 3D structures by two orders of magnitude compared to those previously demonstrated. Finally, functional micro-metric and sub-micrometric patterned features are demonstrated with an antibody binding assay with the recognition of the printed spots with labeled antibodies from solution. The selective addition of physical and chemical attributes on the suspended chips represents the basis for future biomedical assays performed within extremely small volumes. |
| first_indexed | 2025-11-14T20:13:02Z |
| format | Article |
| id | nottingham-49510 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:13:02Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-495102020-05-04T17:36:39Z https://eprints.nottingham.ac.uk/49510/ Highly anisotropic suspended planar-array chips with multidimensional sub-micrometric biomolecular patterns Agusil, Juan Pablo Torras, Núria Duch, Marta Esteve, Jaume Pérez-García, Lluïsa Samitier, Josep Plaza, José A. Suspended planar-array (SPA) chips embody millions of individual miniaturized arrays to work in extremely small volumes. Here, the basis of a robust methodology for the fabrication of SPA silicon chips with on-demand physical and chemical anisotropies is demonstrated. Specifically, physical traits are defined during the fabrication process with special focus on the aspect ratio, branching, faceting, and size gradient of the final chips. Additionally, the chemical attributes augment the functionality of the chips with the inclusion of complete coverage or patterns of selected biomolecules on the surface of the chips with contact printing techniques, offering an extremely high versatility, not only with the choice of the pattern shape and distribution but also in the choice of biomolecular inks to pattern. This approach increases the miniaturization of printed arrays in 3D structures by two orders of magnitude compared to those previously demonstrated. Finally, functional micro-metric and sub-micrometric patterned features are demonstrated with an antibody binding assay with the recognition of the printed spots with labeled antibodies from solution. The selective addition of physical and chemical attributes on the suspended chips represents the basis for future biomedical assays performed within extremely small volumes. Wiley 2017-04-05 Article PeerReviewed Agusil, Juan Pablo, Torras, Núria, Duch, Marta, Esteve, Jaume, Pérez-García, Lluïsa, Samitier, Josep and Plaza, José A. (2017) Highly anisotropic suspended planar-array chips with multidimensional sub-micrometric biomolecular patterns. Advanced Functional Materials, 27 (13). 1605912/1-1605912/11. ISSN 1616-3028 https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201605912 doi:10.1002/adfm.201605912 doi:10.1002/adfm.201605912 |
| spellingShingle | Agusil, Juan Pablo Torras, Núria Duch, Marta Esteve, Jaume Pérez-García, Lluïsa Samitier, Josep Plaza, José A. Highly anisotropic suspended planar-array chips with multidimensional sub-micrometric biomolecular patterns |
| title | Highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| title_full | Highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| title_fullStr | Highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| title_full_unstemmed | Highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| title_short | Highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| title_sort | highly anisotropic suspended planar-array chips with
multidimensional sub-micrometric biomolecular patterns |
| url | https://eprints.nottingham.ac.uk/49510/ https://eprints.nottingham.ac.uk/49510/ https://eprints.nottingham.ac.uk/49510/ |