Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity
Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2017
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| Online Access: | https://eprints.nottingham.ac.uk/49508/ |
| _version_ | 1848798014149230592 |
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| author | Alea-Reyes, Maria E. Rodrigues, Mafalda Serra, Albert Mora, Margarita Sagrista, Maria L. Gonzalez, Asensio Duran, Sara Duch, Marta Plaza, Jose Antonio Valles, Elisa Russell, David A. Pérez-García, Lluïsa |
| author_facet | Alea-Reyes, Maria E. Rodrigues, Mafalda Serra, Albert Mora, Margarita Sagrista, Maria L. Gonzalez, Asensio Duran, Sara Duch, Marta Plaza, Jose Antonio Valles, Elisa Russell, David A. Pérez-García, Lluïsa |
| author_sort | Alea-Reyes, Maria E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of their size and the amount of loaded porphyrin was considered to discuss their effects in the observed photodynamic activity. All the vehicles have a gold surface, allowing functionalization with a disulphide-containing porphyrin as the photosensitizer, as well as with a PEG-containing thiol to improve their biocompatibility and water solubility. The activity of the porphyrin loaded in each vehicle was assessed through in vitro photocytotoxicity studies using HeLa cells. A synergic effect for the porphyrin toxicity was observed in all of the vehicles. The zinc-containing porphyrin showed better production of singlet oxygen, and proved more photocytotoxic both in solution and loaded in any of the vehicles. The magnetism of the nanorods allows targeting with a magnetic field, but causes their aggregation, hampering the porphyrin's activity. Microparticles showed lower cell internalization but their bigger size allowed a high porphyrin loading, which translated into high photocytotoxicity. The highest cell internalization and photocytotoxicity was observed for the porphyrin-loaded nanoparticles, suggesting that a smaller size is favored in cell uptake. |
| first_indexed | 2025-11-14T20:13:02Z |
| format | Article |
| id | nottingham-49508 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:13:02Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-495082020-05-04T18:37:55Z https://eprints.nottingham.ac.uk/49508/ Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity Alea-Reyes, Maria E. Rodrigues, Mafalda Serra, Albert Mora, Margarita Sagrista, Maria L. Gonzalez, Asensio Duran, Sara Duch, Marta Plaza, Jose Antonio Valles, Elisa Russell, David A. Pérez-García, Lluïsa Three nanostructured vehicles are proposed as potential carriers for photosensitizers to be used in photodynamic therapy: spherical nanoparticles, hexahedral microparticles and cylindrical magnetic nanorods. A comparative study of their photodynamic properties was performed, and the influence of their size and the amount of loaded porphyrin was considered to discuss their effects in the observed photodynamic activity. All the vehicles have a gold surface, allowing functionalization with a disulphide-containing porphyrin as the photosensitizer, as well as with a PEG-containing thiol to improve their biocompatibility and water solubility. The activity of the porphyrin loaded in each vehicle was assessed through in vitro photocytotoxicity studies using HeLa cells. A synergic effect for the porphyrin toxicity was observed in all of the vehicles. The zinc-containing porphyrin showed better production of singlet oxygen, and proved more photocytotoxic both in solution and loaded in any of the vehicles. The magnetism of the nanorods allows targeting with a magnetic field, but causes their aggregation, hampering the porphyrin's activity. Microparticles showed lower cell internalization but their bigger size allowed a high porphyrin loading, which translated into high photocytotoxicity. The highest cell internalization and photocytotoxicity was observed for the porphyrin-loaded nanoparticles, suggesting that a smaller size is favored in cell uptake. Royal Society of Chemistry 2017-03-17 Article PeerReviewed Alea-Reyes, Maria E., Rodrigues, Mafalda, Serra, Albert, Mora, Margarita, Sagrista, Maria L., Gonzalez, Asensio, Duran, Sara, Duch, Marta, Plaza, Jose Antonio, Valles, Elisa, Russell, David A. and Pérez-García, Lluïsa (2017) Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity. RSC Advances, 7 . pp. 16963-16976. ISSN 2046-2069 http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C7RA01569K#!divAbstract doi:10.1039/c7ra01569k doi:10.1039/c7ra01569k |
| spellingShingle | Alea-Reyes, Maria E. Rodrigues, Mafalda Serra, Albert Mora, Margarita Sagrista, Maria L. Gonzalez, Asensio Duran, Sara Duch, Marta Plaza, Jose Antonio Valles, Elisa Russell, David A. Pérez-García, Lluïsa Nanostructured materials for photodynamic therapy: synthesis, characterization and in vitro activity |
| title | Nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| title_full | Nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| title_fullStr | Nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| title_full_unstemmed | Nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| title_short | Nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| title_sort | nanostructured materials for photodynamic
therapy: synthesis, characterization and in vitro
activity |
| url | https://eprints.nottingham.ac.uk/49508/ https://eprints.nottingham.ac.uk/49508/ https://eprints.nottingham.ac.uk/49508/ |