LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice

Root system architecture is very important for plant growth and crop yield. It is essential for nutrient and water uptake, anchoring, and mechanical support. Root growth angle (RGA) is a vital constituent of root system architecture and is used as a parameter for variety evaluation in plant breeding...

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Main Authors: Wang, Lingling, Guo, Mengxue, Li, Yong, Ruan, Wenyuan, Mo, Xiaorong, Wu, Zhongchang, Sturrock, Craig, Yu, Hao, Lu, Chungui, Peng, Jinrong, Mao, Chuanzao
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Published: Oxford University Press 2017
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Online Access:https://eprints.nottingham.ac.uk/49147/
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author Wang, Lingling
Guo, Mengxue
Li, Yong
Ruan, Wenyuan
Mo, Xiaorong
Wu, Zhongchang
Sturrock, Craig
Yu, Hao
Lu, Chungui
Peng, Jinrong
Mao, Chuanzao
author_facet Wang, Lingling
Guo, Mengxue
Li, Yong
Ruan, Wenyuan
Mo, Xiaorong
Wu, Zhongchang
Sturrock, Craig
Yu, Hao
Lu, Chungui
Peng, Jinrong
Mao, Chuanzao
author_sort Wang, Lingling
building Nottingham Research Data Repository
collection Online Access
description Root system architecture is very important for plant growth and crop yield. It is essential for nutrient and water uptake, anchoring, and mechanical support. Root growth angle (RGA) is a vital constituent of root system architecture and is used as a parameter for variety evaluation in plant breeding. However, little is known about the underlying molecular mechanisms that determine root growth angle in rice (Oryza sativa). In this study, a rice mutant large root angle1 (lra1) was isolated and shown to exhibit a large RGA and reduced sensitivity to gravity. Genome resequencing and complementation assays identified OsPIN2 as the gene responsible for the mutant phenotypes. OsPIN2 was mainly expressed in roots and the base of shoots, and showed polar localization in the plasma membrane of root epidermal and cortex cells. OsPIN2 was shown to play an important role in mediating root gravitropic responses in rice and was essential for plants to produce normal RGAs. Taken together, our findings suggest that OsPIN2 plays an important role in root gravitropic responses and determining the root system architecture in rice by affecting polar auxin transport in the root tip.
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institution University of Nottingham Malaysia Campus
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publishDate 2017
publisher Oxford University Press
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spelling nottingham-491472020-05-04T19:23:42Z https://eprints.nottingham.ac.uk/49147/ LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice Wang, Lingling Guo, Mengxue Li, Yong Ruan, Wenyuan Mo, Xiaorong Wu, Zhongchang Sturrock, Craig Yu, Hao Lu, Chungui Peng, Jinrong Mao, Chuanzao Root system architecture is very important for plant growth and crop yield. It is essential for nutrient and water uptake, anchoring, and mechanical support. Root growth angle (RGA) is a vital constituent of root system architecture and is used as a parameter for variety evaluation in plant breeding. However, little is known about the underlying molecular mechanisms that determine root growth angle in rice (Oryza sativa). In this study, a rice mutant large root angle1 (lra1) was isolated and shown to exhibit a large RGA and reduced sensitivity to gravity. Genome resequencing and complementation assays identified OsPIN2 as the gene responsible for the mutant phenotypes. OsPIN2 was mainly expressed in roots and the base of shoots, and showed polar localization in the plasma membrane of root epidermal and cortex cells. OsPIN2 was shown to play an important role in mediating root gravitropic responses in rice and was essential for plants to produce normal RGAs. Taken together, our findings suggest that OsPIN2 plays an important role in root gravitropic responses and determining the root system architecture in rice by affecting polar auxin transport in the root tip. Oxford University Press 2017-12-22 Article PeerReviewed Wang, Lingling, Guo, Mengxue, Li, Yong, Ruan, Wenyuan, Mo, Xiaorong, Wu, Zhongchang, Sturrock, Craig, Yu, Hao, Lu, Chungui, Peng, Jinrong and Mao, Chuanzao (2017) LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice. Journal of Experimental Botany . ISSN 1460-2431 Auxin gravitropism OsPIN2 rice root growth angle root system architecture https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erx427/4773865 doi:10.1093/jxb/erx427 doi:10.1093/jxb/erx427
spellingShingle Auxin
gravitropism
OsPIN2
rice
root growth angle
root system architecture
Wang, Lingling
Guo, Mengxue
Li, Yong
Ruan, Wenyuan
Mo, Xiaorong
Wu, Zhongchang
Sturrock, Craig
Yu, Hao
Lu, Chungui
Peng, Jinrong
Mao, Chuanzao
LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title_full LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title_fullStr LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title_full_unstemmed LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title_short LARGE ROOT ANGLE1, encoding OsPIN2, is involved in root system architecture in rice
title_sort large root angle1, encoding ospin2, is involved in root system architecture in rice
topic Auxin
gravitropism
OsPIN2
rice
root growth angle
root system architecture
url https://eprints.nottingham.ac.uk/49147/
https://eprints.nottingham.ac.uk/49147/
https://eprints.nottingham.ac.uk/49147/