An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells
The development of new assays to study trans-plasma membrane electron transport (tPMET) in eukaryotic systems is paramount for a number reasons, which include the further understanding of the underlying biology which can then potentially be applied to innovate technological advancements in biosensin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
American Chemical Society
2018
|
| Online Access: | https://eprints.nottingham.ac.uk/49124/ |
| _version_ | 1848797927366983680 |
|---|---|
| author | Sherman, Harry G. Jovanovic, Carolyn Stolnik, Snow Rawson, Frankie J. |
| author_facet | Sherman, Harry G. Jovanovic, Carolyn Stolnik, Snow Rawson, Frankie J. |
| author_sort | Sherman, Harry G. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The development of new assays to study trans-plasma membrane electron transport (tPMET) in eukaryotic systems is paramount for a number reasons, which include the further understanding of the underlying biology which can then potentially be applied to innovate technological advancements in biosensing, microbial fuel, and pharmaceutical fields. The current literature provides methodology to study these systems that hinges upon mitochondrial knockout genotypes, or the detection of ferrocyanide using colorimetric methods. Developing a method to simultaneously analyze the redox state of a reporter molecule would give advantages in probing the underlying biology. Herein we present an electrochemical based method that allows for the quantification of both ferricyanide and ferrocyanide redox states to a highly sensitive degree. We have applied this system to a novel application of assessing oncogenic cell-driven iron reduction, and have shown that it can effectively quantitate and identify differences in iron reduction capability of three lung epithelial cell lines. Importantly, the development of the technology has led to new biological hypothesis which now need addressing. |
| first_indexed | 2025-11-14T20:11:39Z |
| format | Article |
| id | nottingham-49124 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:11:39Z |
| publishDate | 2018 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-491242020-05-04T19:26:58Z https://eprints.nottingham.ac.uk/49124/ An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells Sherman, Harry G. Jovanovic, Carolyn Stolnik, Snow Rawson, Frankie J. The development of new assays to study trans-plasma membrane electron transport (tPMET) in eukaryotic systems is paramount for a number reasons, which include the further understanding of the underlying biology which can then potentially be applied to innovate technological advancements in biosensing, microbial fuel, and pharmaceutical fields. The current literature provides methodology to study these systems that hinges upon mitochondrial knockout genotypes, or the detection of ferrocyanide using colorimetric methods. Developing a method to simultaneously analyze the redox state of a reporter molecule would give advantages in probing the underlying biology. Herein we present an electrochemical based method that allows for the quantification of both ferricyanide and ferrocyanide redox states to a highly sensitive degree. We have applied this system to a novel application of assessing oncogenic cell-driven iron reduction, and have shown that it can effectively quantitate and identify differences in iron reduction capability of three lung epithelial cell lines. Importantly, the development of the technology has led to new biological hypothesis which now need addressing. American Chemical Society 2018-01-15 Article PeerReviewed Sherman, Harry G., Jovanovic, Carolyn, Stolnik, Snow and Rawson, Frankie J. (2018) An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells. Analytical Chemistry, 90 (4). pp. 2780-2786. ISSN 1520-6882 http://pubs.acs.org/doi/10.1021/acs.analchem.7b04853 doi:10.1021/acs.analchem.7b04853 doi:10.1021/acs.analchem.7b04853 |
| spellingShingle | Sherman, Harry G. Jovanovic, Carolyn Stolnik, Snow Rawson, Frankie J. An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title | An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title_full | An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title_fullStr | An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title_full_unstemmed | An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title_short | An electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| title_sort | electrochemical system for the study of trans-plasma membrane electron transport in whole eukaryotic cells |
| url | https://eprints.nottingham.ac.uk/49124/ https://eprints.nottingham.ac.uk/49124/ https://eprints.nottingham.ac.uk/49124/ |