Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation
The ability of materials to define the architecture and micro-environment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) (Robinton DA, et al (2012) Nature 81:295-305). However, the conditions required for self-renewal verses differentiation...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences
2014
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| Online Access: | https://eprints.nottingham.ac.uk/49049/ |
| _version_ | 1848797911078404096 |
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| author | Dixon, James E. Shah, Disheet A. Rogers, Catherine Hall, Stephen Weston, Nicola Parmenter, Christopher D. J. McNally, Donal Denning, Chris Shakesheff, Kevin M. |
| author_facet | Dixon, James E. Shah, Disheet A. Rogers, Catherine Hall, Stephen Weston, Nicola Parmenter, Christopher D. J. McNally, Donal Denning, Chris Shakesheff, Kevin M. |
| author_sort | Dixon, James E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The ability of materials to define the architecture and micro-environment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) (Robinton DA, et al (2012) Nature 81:295-305). However, the conditions required for self-renewal verses differentiation of HPSCs are different and a single system that efficiently achieves both outcomes is not available (Giobbe GG, et al. (2012) Biotech Bioeng 109:3119 - 3132). We have addressed this dual need by developing a hydrogel - based material that uses ionic decrosslinking to remove a self-renewal permissive hydrogel (alginate) and switch to a differentiation-permissive micro-environment (collagen). Adjusting the timing of this switch can preferentially steer the HPSC differentiation to mimic lineage commitment during gastrulation to ectoderm (early switch) or mesoderm/endoderm (late switch). As an exemplar differentiated cell type, we showed that directing early-lineage specification using this single system can promote cardiogenesis with increased gene expression in high-density cell populations. This work will facilitate regenerative medicine by allowing in situ HPSC expansion to be coupled with early lineage-specification within defined tissue geometries. |
| first_indexed | 2025-11-14T20:11:23Z |
| format | Article |
| id | nottingham-49049 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:11:23Z |
| publishDate | 2014 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-490492020-05-08T09:30:20Z https://eprints.nottingham.ac.uk/49049/ Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation Dixon, James E. Shah, Disheet A. Rogers, Catherine Hall, Stephen Weston, Nicola Parmenter, Christopher D. J. McNally, Donal Denning, Chris Shakesheff, Kevin M. The ability of materials to define the architecture and micro-environment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) (Robinton DA, et al (2012) Nature 81:295-305). However, the conditions required for self-renewal verses differentiation of HPSCs are different and a single system that efficiently achieves both outcomes is not available (Giobbe GG, et al. (2012) Biotech Bioeng 109:3119 - 3132). We have addressed this dual need by developing a hydrogel - based material that uses ionic decrosslinking to remove a self-renewal permissive hydrogel (alginate) and switch to a differentiation-permissive micro-environment (collagen). Adjusting the timing of this switch can preferentially steer the HPSC differentiation to mimic lineage commitment during gastrulation to ectoderm (early switch) or mesoderm/endoderm (late switch). As an exemplar differentiated cell type, we showed that directing early-lineage specification using this single system can promote cardiogenesis with increased gene expression in high-density cell populations. This work will facilitate regenerative medicine by allowing in situ HPSC expansion to be coupled with early lineage-specification within defined tissue geometries. National Academy of Sciences 2014-04-15 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/49049/1/Dixon%20et%20al.%20PNAS%202014%20ePrints.pdf Dixon, James E., Shah, Disheet A., Rogers, Catherine, Hall, Stephen, Weston, Nicola, Parmenter, Christopher D. J., McNally, Donal, Denning, Chris and Shakesheff, Kevin M. (2014) Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111 (15). pp. 5580-5585. ISSN 1091-6490 Human Embryonic Stem Cells Differentiation Hydrogel Cardiomyocyte http://www.pnas.org/content/111/15/5580 doi:10.1073/pnas.1319685111 doi:10.1073/pnas.1319685111 |
| spellingShingle | Human Embryonic Stem Cells Differentiation Hydrogel Cardiomyocyte Dixon, James E. Shah, Disheet A. Rogers, Catherine Hall, Stephen Weston, Nicola Parmenter, Christopher D. J. McNally, Donal Denning, Chris Shakesheff, Kevin M. Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title | Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title_full | Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title_fullStr | Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title_full_unstemmed | Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title_short | Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| title_sort | combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation |
| topic | Human Embryonic Stem Cells Differentiation Hydrogel Cardiomyocyte |
| url | https://eprints.nottingham.ac.uk/49049/ https://eprints.nottingham.ac.uk/49049/ https://eprints.nottingham.ac.uk/49049/ |