Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review)
Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to widescale ado...
| Main Authors: | , |
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| Format: | Article |
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AIP Publishing
2018
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| Online Access: | https://eprints.nottingham.ac.uk/48846/ |
| _version_ | 1848797862295502848 |
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| author | Harrison, Richard P. Chauhan, Veeren M. |
| author_facet | Harrison, Richard P. Chauhan, Veeren M. |
| author_sort | Harrison, Richard P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to widescale adoption of CGTs remains challenging, such that the emergence of a “blockbuster” therapy has so far proved elusive. Manufacturing solutions for these therapies require the application of scalable and replicable cell manufacturing techniques, which differ markedly from the existing pharmaceutical incumbent. Attempts to adopt this pharmaceutical model for CGT manufacture have largely proved unsuccessful. The most significant challenges facing CGT manufacturing are process analytical testing and quality control. These procedures would greatly benefit from improved sensory technologies that allow direct measurement of critical quality attributes, such as pH, oxygen, lactate and glucose. In turn, this would make manufacturing more robust, replicable and standardized. In this review, the present-day state and prospects of CGT manufacturing are discussed. In particular, the authors highlight the role of fluorescent optical sensors, focusing on their strengths and weaknesses, for CGT manufacture. The review concludes by discussing how the integration of CGT manufacture and fluorescent optical sensors could augment future bioprocessing approaches. |
| first_indexed | 2025-11-14T20:10:37Z |
| format | Article |
| id | nottingham-48846 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:10:37Z |
| publishDate | 2018 |
| publisher | AIP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-488462020-05-04T19:53:03Z https://eprints.nottingham.ac.uk/48846/ Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) Harrison, Richard P. Chauhan, Veeren M. Cell and gene therapies (CGTs) are examples of future therapeutics that can be used to cure or alleviate the symptoms of disease, by repairing damaged tissue or reprogramming defective genetic information. However, despite the recent advancements in clinical trial outcomes, the path to widescale adoption of CGTs remains challenging, such that the emergence of a “blockbuster” therapy has so far proved elusive. Manufacturing solutions for these therapies require the application of scalable and replicable cell manufacturing techniques, which differ markedly from the existing pharmaceutical incumbent. Attempts to adopt this pharmaceutical model for CGT manufacture have largely proved unsuccessful. The most significant challenges facing CGT manufacturing are process analytical testing and quality control. These procedures would greatly benefit from improved sensory technologies that allow direct measurement of critical quality attributes, such as pH, oxygen, lactate and glucose. In turn, this would make manufacturing more robust, replicable and standardized. In this review, the present-day state and prospects of CGT manufacturing are discussed. In particular, the authors highlight the role of fluorescent optical sensors, focusing on their strengths and weaknesses, for CGT manufacture. The review concludes by discussing how the integration of CGT manufacture and fluorescent optical sensors could augment future bioprocessing approaches. AIP Publishing 2018-01 Article PeerReviewed Harrison, Richard P. and Chauhan, Veeren M. (2018) Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review). Biointerphases, 13 (1). 01A301-1-01A301-8. ISSN 1559-4106 http://avs.scitation.org/doi/10.1116/1.5013335 doi:10.1116/1.5013335 doi:10.1116/1.5013335 |
| spellingShingle | Harrison, Richard P. Chauhan, Veeren M. Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title | Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title_full | Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title_fullStr | Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title_full_unstemmed | Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title_short | Enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (Review) |
| title_sort | enhancing cell and gene therapy manufacture through the application of advanced fluorescent optical sensors (review) |
| url | https://eprints.nottingham.ac.uk/48846/ https://eprints.nottingham.ac.uk/48846/ https://eprints.nottingham.ac.uk/48846/ |