Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry
Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as toxoplasmosis encephalitis and toxoplasmic chorioretinitis. This study aimed to determine whether serum metabolic profiling can (i) identify me...
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Frontiers Media
2018
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| Online Access: | https://eprints.nottingham.ac.uk/48831/ |
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| author | Zhou, Chun-Xue Cong, Wei Chen, Xiao-Qing He, Shen-Yi Elsheikha, Hany M. Zhu, Xing-Quan |
| author_facet | Zhou, Chun-Xue Cong, Wei Chen, Xiao-Qing He, Shen-Yi Elsheikha, Hany M. Zhu, Xing-Quan |
| author_sort | Zhou, Chun-Xue |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as toxoplasmosis encephalitis and toxoplasmic chorioretinitis. This study aimed to determine whether serum metabolic profiling can (i) identify metabolites associated with oocyst-induced T. gondii infection and (ii) detect systemic metabolic differences between T. gondii -infected mice patients and controls. We performed the first global metabolomics analysis of mice serum challenged with 100 sporulated T. gondii Pru oocysts (Genotype II). Sera from acutely infected mice (11 days post-infection, dpi), chronically infected mice (33 dpi) and control mice were collected and analysed using LC-MS/MS platform. Following False Discovery Rate filtering, we identified 3871 and 2825 ions in ESI + or ESI − mode, respectively. Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS -DA) identified metabolomics profiles that clearly differentiated T. gondii -infected and -uninfected serum samples. Acute infection significantly influenced the serum metabolonme. Our results identified common and uniquely perturbed metabolites and pathways. Acutely infected mice showed perturbations in metabolites associated with glycerophospholipid metabolism, biosynthesis of amino acid, and tyrosine metabolism. These findings demonstrated that acute T. gondii oocyst induces a global perturbation of mice serum metabolonme, providing new insights into the mechanisms underlying systemic metabolic changes during early stage of T. gondii oocyst infection. |
| first_indexed | 2025-11-14T20:10:34Z |
| format | Article |
| id | nottingham-48831 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:10:34Z |
| publishDate | 2018 |
| publisher | Frontiers Media |
| recordtype | eprints |
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| spelling | nottingham-488312020-05-04T19:25:55Z https://eprints.nottingham.ac.uk/48831/ Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry Zhou, Chun-Xue Cong, Wei Chen, Xiao-Qing He, Shen-Yi Elsheikha, Hany M. Zhu, Xing-Quan Toxoplasma gondii is an obligate intracellular parasite causing severe diseases in immunocompromised individuals and congenitally infected neonates, such as toxoplasmosis encephalitis and toxoplasmic chorioretinitis. This study aimed to determine whether serum metabolic profiling can (i) identify metabolites associated with oocyst-induced T. gondii infection and (ii) detect systemic metabolic differences between T. gondii -infected mice patients and controls. We performed the first global metabolomics analysis of mice serum challenged with 100 sporulated T. gondii Pru oocysts (Genotype II). Sera from acutely infected mice (11 days post-infection, dpi), chronically infected mice (33 dpi) and control mice were collected and analysed using LC-MS/MS platform. Following False Discovery Rate filtering, we identified 3871 and 2825 ions in ESI + or ESI − mode, respectively. Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS -DA) identified metabolomics profiles that clearly differentiated T. gondii -infected and -uninfected serum samples. Acute infection significantly influenced the serum metabolonme. Our results identified common and uniquely perturbed metabolites and pathways. Acutely infected mice showed perturbations in metabolites associated with glycerophospholipid metabolism, biosynthesis of amino acid, and tyrosine metabolism. These findings demonstrated that acute T. gondii oocyst induces a global perturbation of mice serum metabolonme, providing new insights into the mechanisms underlying systemic metabolic changes during early stage of T. gondii oocyst infection. Frontiers Media 2018-01-04 Article PeerReviewed Zhou, Chun-Xue, Cong, Wei, Chen, Xiao-Qing, He, Shen-Yi, Elsheikha, Hany M. and Zhu, Xing-Quan (2018) Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry. Frontiers in Microbiology . ISSN 1664-302X Toxoplasma gondii oocyst LC-MS/MS metabolomics mouse model https://www.frontiersin.org/articles/10.3389/fmicb.2017.02612/full doi:10.3389/fmicb.2017.02612 doi:10.3389/fmicb.2017.02612 |
| spellingShingle | Toxoplasma gondii oocyst LC-MS/MS metabolomics mouse model Zhou, Chun-Xue Cong, Wei Chen, Xiao-Qing He, Shen-Yi Elsheikha, Hany M. Zhu, Xing-Quan Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title | Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title_full | Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title_fullStr | Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title_full_unstemmed | Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title_short | Serum metabolic profiling of oocyst-induced Toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| title_sort | serum metabolic profiling of oocyst-induced toxoplasma gondii acute and chronic infections in mice using mass-spectrometry |
| topic | Toxoplasma gondii oocyst LC-MS/MS metabolomics mouse model |
| url | https://eprints.nottingham.ac.uk/48831/ https://eprints.nottingham.ac.uk/48831/ https://eprints.nottingham.ac.uk/48831/ |