Candida albicans Hap43 domains are required under iron starvation but not excess

Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tightly coordinate iron homeostasis in response to cha...

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Main Authors: Skrahina, Volha, Brock, Matthias, Hube, Bernhard, Brunke, Sascha
Format: Article
Published: Frontiers Media 2017
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Online Access:https://eprints.nottingham.ac.uk/48794/
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author Skrahina, Volha
Brock, Matthias
Hube, Bernhard
Brunke, Sascha
author_facet Skrahina, Volha
Brock, Matthias
Hube, Bernhard
Brunke, Sascha
author_sort Skrahina, Volha
building Nottingham Research Data Repository
collection Online Access
description Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tightly coordinate iron homeostasis in response to changing iron levels. As a commensal and opportunistic pathogen, Candida albicans copes with both iron deficiency and excess via the precise regulation of iron acquisition, consumption and storage. The C. albicans transcription factor Hap43 is known to be required for the iron starvation response, while specific domains of its ortholog, HapX, in Aspergillus fumigatus, were recently shown to regulate iron uptake and consumptions genes under both low and high iron levels. Therefore, we investigated the contribution of C. albicans Hap43 domains in response to changing iron levels. We found the C-terminus of Hap43 to be essential for the activation of iron uptake genes during iron starvation, whereas, in contrast to A. fumigatus, Hap43 was not required in mediating adaptation to iron resistance. These data indicate that the generally conserved metal acquisition systems in fungal pathogens can show individual adaptations to the host environment.
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spelling nottingham-487942020-05-04T19:20:52Z https://eprints.nottingham.ac.uk/48794/ Candida albicans Hap43 domains are required under iron starvation but not excess Skrahina, Volha Brock, Matthias Hube, Bernhard Brunke, Sascha Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tightly coordinate iron homeostasis in response to changing iron levels. As a commensal and opportunistic pathogen, Candida albicans copes with both iron deficiency and excess via the precise regulation of iron acquisition, consumption and storage. The C. albicans transcription factor Hap43 is known to be required for the iron starvation response, while specific domains of its ortholog, HapX, in Aspergillus fumigatus, were recently shown to regulate iron uptake and consumptions genes under both low and high iron levels. Therefore, we investigated the contribution of C. albicans Hap43 domains in response to changing iron levels. We found the C-terminus of Hap43 to be essential for the activation of iron uptake genes during iron starvation, whereas, in contrast to A. fumigatus, Hap43 was not required in mediating adaptation to iron resistance. These data indicate that the generally conserved metal acquisition systems in fungal pathogens can show individual adaptations to the host environment. Frontiers Media 2017-12-01 Article PeerReviewed Skrahina, Volha, Brock, Matthias, Hube, Bernhard and Brunke, Sascha (2017) Candida albicans Hap43 domains are required under iron starvation but not excess. Frontiers in Microbiology, 8 . 2388/1-2388/10. ISSN 1664-302X iron homeostasis iron deficiency and toxicity transcriptional regulation hemoglobin fungal pathogenicity https://www.frontiersin.org/articles/10.3389/fmicb.2017.02388/full doi:10.3389/fmicb.2017.02388 doi:10.3389/fmicb.2017.02388
spellingShingle iron homeostasis
iron deficiency and toxicity
transcriptional regulation
hemoglobin
fungal pathogenicity
Skrahina, Volha
Brock, Matthias
Hube, Bernhard
Brunke, Sascha
Candida albicans Hap43 domains are required under iron starvation but not excess
title Candida albicans Hap43 domains are required under iron starvation but not excess
title_full Candida albicans Hap43 domains are required under iron starvation but not excess
title_fullStr Candida albicans Hap43 domains are required under iron starvation but not excess
title_full_unstemmed Candida albicans Hap43 domains are required under iron starvation but not excess
title_short Candida albicans Hap43 domains are required under iron starvation but not excess
title_sort candida albicans hap43 domains are required under iron starvation but not excess
topic iron homeostasis
iron deficiency and toxicity
transcriptional regulation
hemoglobin
fungal pathogenicity
url https://eprints.nottingham.ac.uk/48794/
https://eprints.nottingham.ac.uk/48794/
https://eprints.nottingham.ac.uk/48794/