Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells
Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride c...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Wiley Open Access
2018
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| Online Access: | https://eprints.nottingham.ac.uk/48775/ |
| _version_ | 1848797843390726144 |
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| author | Liu, Bo Billington, Charlotte K. Henry, Amanda P. Bhaker, Sangita K. Kheirallah, Alexander K. Swan, Caroline Hall, Ian P. |
| author_facet | Liu, Bo Billington, Charlotte K. Henry, Amanda P. Bhaker, Sangita K. Kheirallah, Alexander K. Swan, Caroline Hall, Ian P. |
| author_sort | Liu, Bo |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterise the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP-induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type. |
| first_indexed | 2025-11-14T20:10:19Z |
| format | Article |
| id | nottingham-48775 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:10:19Z |
| publishDate | 2018 |
| publisher | Wiley Open Access |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-487752020-05-04T19:28:00Z https://eprints.nottingham.ac.uk/48775/ Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells Liu, Bo Billington, Charlotte K. Henry, Amanda P. Bhaker, Sangita K. Kheirallah, Alexander K. Swan, Caroline Hall, Ian P. Chloride channels are known to play critical physiological roles in many cell types. Here we describe the expression of anion channels using RNA Seq in primary cultures of human Bronchial Epithelial Cells (hBECs). Chloride intracellular channel (CLIC) family members were the most abundant chloride channel transcripts, and CLIC1 showed the highest level of expression. In addition, we characterise the chloride currents in hBECs and determine how inhibition of CLIC1 via pharmacological and molecular approaches impacts these. We demonstrate that CLIC1 is able to modulate cyclic AMP-induced chloride currents and suggest that CLIC1 modulation could be important for chloride homeostasis in this cell type. Wiley Open Access 2018-01-25 Article PeerReviewed Liu, Bo, Billington, Charlotte K., Henry, Amanda P., Bhaker, Sangita K., Kheirallah, Alexander K., Swan, Caroline and Hall, Ian P. (2018) Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells. Physiological Reports, 6 (2). e13508. ISSN 2051-817X Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP http://physreports.physiology.org/content/6/2/e13508 doi:10.14814/phy2.13508 doi:10.14814/phy2.13508 |
| spellingShingle | Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP Liu, Bo Billington, Charlotte K. Henry, Amanda P. Bhaker, Sangita K. Kheirallah, Alexander K. Swan, Caroline Hall, Ian P. Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title | Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title_full | Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title_fullStr | Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title_full_unstemmed | Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title_short | Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP-activated whole cell chloride currents in human bronchial epithelial cells |
| title_sort | chloride intracellular channel 1 (clic1) contributes to modulation of cyclic amp-activated whole cell chloride currents in human bronchial epithelial cells |
| topic | Human bronchial epithelial cells; chloride channels; CLIC1; cyclic AMP |
| url | https://eprints.nottingham.ac.uk/48775/ https://eprints.nottingham.ac.uk/48775/ https://eprints.nottingham.ac.uk/48775/ |