Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival
Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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ASM Press
2005
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| Online Access: | https://eprints.nottingham.ac.uk/48702/ |
| _version_ | 1848797827618045952 |
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| author | Griffin, R. Bayliss, Christopher D. Herbert, Mark A. Cox, Andrew D. Makepeace, Katherine Richards, J.C. Hood, Derek W. Moxon, E. Richard |
| author_facet | Griffin, R. Bayliss, Christopher D. Herbert, Mark A. Cox, Andrew D. Makepeace, Katherine Richards, J.C. Hood, Derek W. Moxon, E. Richard |
| author_sort | Griffin, R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo. |
| first_indexed | 2025-11-14T20:10:04Z |
| format | Article |
| id | nottingham-48702 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:10:04Z |
| publishDate | 2005 |
| publisher | ASM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-487022020-05-04T16:26:11Z https://eprints.nottingham.ac.uk/48702/ Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival Griffin, R. Bayliss, Christopher D. Herbert, Mark A. Cox, Andrew D. Makepeace, Katherine Richards, J.C. Hood, Derek W. Moxon, E. Richard Digalactoside (gal-1-4 gal) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo. ASM Press 2005-10-01 Article PeerReviewed Griffin, R., Bayliss, Christopher D., Herbert, Mark A., Cox, Andrew D., Makepeace, Katherine, Richards, J.C., Hood, Derek W. and Moxon, E. Richard (2005) Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival. Infection and Immunity, 73 (10). pp. 7022-7026. ISSN 1098-5522 http://iai.asm.org/content/73/10/7022 doi:10.1128/IAI.73.10.7022-7026.2005 doi:10.1128/IAI.73.10.7022-7026.2005 |
| spellingShingle | Griffin, R. Bayliss, Christopher D. Herbert, Mark A. Cox, Andrew D. Makepeace, Katherine Richards, J.C. Hood, Derek W. Moxon, E. Richard Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title | Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title_full | Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title_fullStr | Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title_full_unstemmed | Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title_short | Digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| title_sort | digalactoside expression in the lipopolysaccharide of haemophilus influenzae and its role in intravascular survival |
| url | https://eprints.nottingham.ac.uk/48702/ https://eprints.nottingham.ac.uk/48702/ https://eprints.nottingham.ac.uk/48702/ |