Signature-tagged transposon mutagenesis identifies novel
mycobacterium tuberculosis genes involved in the parasitism of human macrophages

QR Code

Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characte...

Full description

Bibliographic Details
Main Authors:	Rosas-Magallanes, Vania, Stadthagen-Gomez, Gustavo, Rauzier, Jean, Barreiro, Luis B., Tailleux, Ludovic, Boudou, Frédéric, Griffin, Ruth, Nigou, Jérome, Jackson, Mary, Gicquel, Brigittte, Neyrolles, Olivier
Format:	Article
Published:	ASM Press 2007
Online Access:	https://eprints.nottingham.ac.uk/48696/

Similar Items

Transposon mutagenesis in RT 078 Clostridioides difficile
by: te Vrugt, Marcel
Published: (2023)

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
by: Zhang, Ying, et al.
Published: (2016)

A plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria
by: Monson, R., et al.
Published: (2015)

p-Hydroxybenzoic acid synthesis in mycobacterium tuberculosis
by: Stadthagen, Gustavo, et al.
Published: (2005)

Comparative investigation of the pathogenicity of three Mycobacterium tuberculosis mutants defective in the synthesis of p-hydroxybenzoic acid derivatives
by: Stadthagen, Gustavo, et al.
Published: (2006)

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis
by: Liu, Danxu, et al.
Published: (2014)

Development of Hsmar1 transposon tools for research and biotechnology
by: Bhatt, Shivam
Published: (2019)

Hsmar1 transposition is sensitive to the topology of the transposon donor and the target
by: Claeys Bouuaert, Corentin, et al.
Published: (2013)

Crosstalk between transposase subunits during cleavage of the mariner transposon
by: Claeys Bouuaert, Corentin, et al.
Published: (2014)

Ant-1, an active transposon from the fungus Aspergillus niger
by: Glayzer, D., et al.
Published: (1995)

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System
by: Blanchard, Adam M., et al.
Published: (2015)

Mobile mercury resistance transposons: surveillance and resistance gene cassette variation in wastewater
by: Pritchard, Alexander C W
Published: (2022)

A new approach to mutagenesis in Clostridium acetobutylicum
by: Furness, Lindsey
Published: (2017)

Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation
by: Chan, Soo Khim
Published: (2018)

Construction of Streptococcus pyogenes mutants by allele replacement mutagenesis
by: Shahdan, Intan Azura, et al.
Published: (2010)

Phytochemical and mutagenesis studies of neolamarckia cadamba and leucaena leucocephala
by: Mohamed Zaky Abdel-Latif, Zayed Sadawe
Published: (2015)

A review on induced mutagenesis of Stevia rebaudiana Bertoni
by: Chiew, Miao Si, et al.
Published: (2016)

Site-directed mutagenesis of superfolder green fluorescent protein
by: Lee, Sock Im
Published: (2015)

A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis
by: Thambyrajah, Roshana Sutharshini, et al.
Published: (2016)

A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis.
by: Thambyrajah, Roshana Sutharshini, et al.
Published: (2016)

The immune response to Mycobacterium avium induced in avian and human macrophage-like cell lines and in chickens
by: Shukur, Mohammed Shukri
Published: (2016)

Proteoliposomes from mycobacterium bovis BCG and mycobacterium smegmatis as new vaccine candidates against tuberculosis
by: Kadir, Ramlah
Published: (2016)

Mutagenesis on cyanobacteria for high CO2 uptake: a review
by: Shari, Ummi S. Halmi, et al.
Published: (2014)

Site-directed mutagenesis on plasmid using polymerase chain reaction
by: Ahmad, Abdul Aziz, et al.
Published: (2018)

The use of random mutagenesis in the functional annotation of the Streptococcus uberis genome
by: Blanchard, Adam
Published: (2015)

Mutagenesis of the nucleocapsid protein of nipah virus involved in capsid assembly.
by: Swee, Tin Ong, et al.
Published: (2009)

In vitro mutagenesis and somaclonal variation in banana to increase drought tolerance
by: Shirani Bidabadi, Siamak
Published: (2011)

Principle and application of plant mutagenesis in crop improvement: a review
by: Oladosu, Yusuff, et al.
Published: (2016)

Site-directed mutagenesis of helical lid of J15 lipase
by: Ho, Chern Min
Published: (2015)

Evaluation of the potential of Mycobacterium smegmatis as vaccine Candidate against tuberculosis by in silico and in vivo studies
by: Nguyen Thi, Le Thuy, et al.
Published: (2010)

Cloning and expression of hypothetical protein targets in burkholderia pseudomallei by Transposon-Directed Insertion Site Sequencing (TraDIS) technique
by: Drahaman, Siti Marhamah, et al.
Published: (2016)

Epidemiological study on Mycobacterium tuberculosis complex and Mycobacterium avium complex in free-range and captive wildlife in Malaysia
by: Madaki, Lekko Yusuf
Published: (2022)

Electrochemical immunosensor for detection of Mycobacterium tuberculosis
by: Mohd Azmi, Umi Zulaikha, et al.
Published: (2018)

The transposon-like correia elements encode numerous strong promoters and provide a potential new mechanism for phase variation in the meningococcus
by: Siddique, Azeem, et al.
Published: (2011)

Cloning and sequencing of an ampicillin resistance gene of a multiple antibiotic resistance transposon from Salmonella typhi / Wong Hann Ling.
by: Wong, Hann Ling
Published: (1999)

The prevalence and potential mobility of Tn21-like mercury resistance transposons in E. coli isolated from dairy cattle slurry
by: Iles, Ethan
Published: (2019)

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis
by: Liew, Fungmin, et al.
Published: (2016)

Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase
by: Abdul Hadi, Muaz, et al.
Published: (2010)

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
by: Hasnain, Hussain, et al.
Published: (2016)

A review on microbial mutagenesis through gamma irradiation for agricultural applications
by: Phua, Choo Kwai Hoe, et al.
Published: (2016)