Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characte...

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Main Authors: Rosas-Magallanes, Vania, Stadthagen-Gomez, Gustavo, Rauzier, Jean, Barreiro, Luis B., Tailleux, Ludovic, Boudou, Frédéric, Griffin, Ruth, Nigou, Jérome, Jackson, Mary, Gicquel, Brigittte, Neyrolles, Olivier
Format: Article
Published: ASM Press 2007
Online Access:https://eprints.nottingham.ac.uk/48696/
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author Rosas-Magallanes, Vania
Stadthagen-Gomez, Gustavo
Rauzier, Jean
Barreiro, Luis B.
Tailleux, Ludovic
Boudou, Frédéric
Griffin, Ruth
Nigou, Jérome
Jackson, Mary
Gicquel, Brigittte
Neyrolles, Olivier
author_facet Rosas-Magallanes, Vania
Stadthagen-Gomez, Gustavo
Rauzier, Jean
Barreiro, Luis B.
Tailleux, Ludovic
Boudou, Frédéric
Griffin, Ruth
Nigou, Jérome
Jackson, Mary
Gicquel, Brigittte
Neyrolles, Olivier
author_sort Rosas-Magallanes, Vania
building Nottingham Research Data Repository
collection Online Access
description Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.
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institution University of Nottingham Malaysia Campus
institution_category Local University
last_indexed 2025-11-14T20:10:02Z
publishDate 2007
publisher ASM Press
recordtype eprints
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spelling nottingham-486962020-05-04T16:26:42Z https://eprints.nottingham.ac.uk/48696/ Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages Rosas-Magallanes, Vania Stadthagen-Gomez, Gustavo Rauzier, Jean Barreiro, Luis B. Tailleux, Ludovic Boudou, Frédéric Griffin, Ruth Nigou, Jérome Jackson, Mary Gicquel, Brigittte Neyrolles, Olivier Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells. ASM Press 2007-01-01 Article PeerReviewed Rosas-Magallanes, Vania, Stadthagen-Gomez, Gustavo, Rauzier, Jean, Barreiro, Luis B., Tailleux, Ludovic, Boudou, Frédéric, Griffin, Ruth, Nigou, Jérome, Jackson, Mary, Gicquel, Brigittte and Neyrolles, Olivier (2007) Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages. Infection and Immunity, 75 (1). 504 -507. ISSN 1098-5522 http://iai.asm.org/content/75/1/504 doi:10.1128/IAI.00058-06. doi:10.1128/IAI.00058-06.
spellingShingle Rosas-Magallanes, Vania
Stadthagen-Gomez, Gustavo
Rauzier, Jean
Barreiro, Luis B.
Tailleux, Ludovic
Boudou, Frédéric
Griffin, Ruth
Nigou, Jérome
Jackson, Mary
Gicquel, Brigittte
Neyrolles, Olivier
Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title_full Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title_fullStr Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title_full_unstemmed Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title_short Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
title_sort signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages
url https://eprints.nottingham.ac.uk/48696/
https://eprints.nottingham.ac.uk/48696/
https://eprints.nottingham.ac.uk/48696/