Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characte...

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Main Authors: Rosas-Magallanes, Vania, Stadthagen-Gomez, Gustavo, Rauzier, Jean, Barreiro, Luis B., Tailleux, Ludovic, Boudou, Frédéric, Griffin, Ruth, Nigou, Jérome, Jackson, Mary, Gicquel, Brigittte, Neyrolles, Olivier
Format: Article
Published: ASM Press 2007
Online Access:https://eprints.nottingham.ac.uk/48696/
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Summary:Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.