The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway
In Arabidopsis, the F-box HAWAIIAN SKIRT (HWS) protein is important for organ growth. Loss of function of HWS exhibits pleiotropic phenotypes including sepal fusion. To dissect the HWS role, we EMS-mutagenized hws-1 seeds and screened for mutations that suppress hws-1 associated phenotypes. We ident...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Public Library of Science
2017
|
| Online Access: | https://eprints.nottingham.ac.uk/48648/ |
| _version_ | 1848797814808641536 |
|---|---|
| author | Zhang, Xuebin Jayaweera, Dasuni Peters, Janny L. Szecsi, Judit Bendahmane, Mohammed Roberts, Jeremy A. Gonzalez-Carranza, Zinnia H. |
| author_facet | Zhang, Xuebin Jayaweera, Dasuni Peters, Janny L. Szecsi, Judit Bendahmane, Mohammed Roberts, Jeremy A. Gonzalez-Carranza, Zinnia H. |
| author_sort | Zhang, Xuebin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In Arabidopsis, the F-box HAWAIIAN SKIRT (HWS) protein is important for organ growth. Loss of function of HWS exhibits pleiotropic phenotypes including sepal fusion. To dissect the HWS role, we EMS-mutagenized hws-1 seeds and screened for mutations that suppress hws-1 associated phenotypes. We identified shs-2 and shs-3 (suppressor of hws-2 and 3) mutants in which the sepal fusion phenotype of hws-1 was suppressed. shs-2 and shs-3 (renamed hst-23/hws-1 and hst-24/hws-1) carry transition mutations that result in premature terminations in the plant homolog of Exportin-5 HASTY (HST), known to be important in miRNA biogenesis, function and transport. Genetic crosses between hws-1 and mutant lines for genes in the miRNA pathway, also suppress the phenotypes associated with HWS loss of function, corroborating epistatic relations between the miRNA pathway genes and HWS. In agreement with these data, accumulation of miRNA is modified in HWS loss or gain of function mutants. Our data propose HWS as a new player in the miRNA pathway, important for plant growth. |
| first_indexed | 2025-11-14T20:09:52Z |
| format | Article |
| id | nottingham-48648 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:09:52Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-486482020-05-04T19:22:48Z https://eprints.nottingham.ac.uk/48648/ The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway Zhang, Xuebin Jayaweera, Dasuni Peters, Janny L. Szecsi, Judit Bendahmane, Mohammed Roberts, Jeremy A. Gonzalez-Carranza, Zinnia H. In Arabidopsis, the F-box HAWAIIAN SKIRT (HWS) protein is important for organ growth. Loss of function of HWS exhibits pleiotropic phenotypes including sepal fusion. To dissect the HWS role, we EMS-mutagenized hws-1 seeds and screened for mutations that suppress hws-1 associated phenotypes. We identified shs-2 and shs-3 (suppressor of hws-2 and 3) mutants in which the sepal fusion phenotype of hws-1 was suppressed. shs-2 and shs-3 (renamed hst-23/hws-1 and hst-24/hws-1) carry transition mutations that result in premature terminations in the plant homolog of Exportin-5 HASTY (HST), known to be important in miRNA biogenesis, function and transport. Genetic crosses between hws-1 and mutant lines for genes in the miRNA pathway, also suppress the phenotypes associated with HWS loss of function, corroborating epistatic relations between the miRNA pathway genes and HWS. In agreement with these data, accumulation of miRNA is modified in HWS loss or gain of function mutants. Our data propose HWS as a new player in the miRNA pathway, important for plant growth. Public Library of Science 2017-12-15 Article PeerReviewed Zhang, Xuebin, Jayaweera, Dasuni, Peters, Janny L., Szecsi, Judit, Bendahmane, Mohammed, Roberts, Jeremy A. and Gonzalez-Carranza, Zinnia H. (2017) The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. PLoS ONE, 12 (12). e0189788/1-e0189788/17. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189788 doi:10.1371/journal.pone.0189788 doi:10.1371/journal.pone.0189788 |
| spellingShingle | Zhang, Xuebin Jayaweera, Dasuni Peters, Janny L. Szecsi, Judit Bendahmane, Mohammed Roberts, Jeremy A. Gonzalez-Carranza, Zinnia H. The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title | The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title_full | The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title_fullStr | The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title_full_unstemmed | The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title_short | The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway |
| title_sort | arabidopsis thaliana f-box gene hawaiian skirt is a new player in the microrna pathway |
| url | https://eprints.nottingham.ac.uk/48648/ https://eprints.nottingham.ac.uk/48648/ https://eprints.nottingham.ac.uk/48648/ |