Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation

A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after lo...

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Main Authors: Celiz, Adam D., Smith, James G.W., Patel, Asha K., Hook, Andrew L., Rajamohan, Divya, George, Vinoj T., Flatt, Luke, Patel, Minal J., Epa, Vidana C., Singh, Taranjit, Langer, Robert, Anderson, Daniel G., Allen, Nicholas D., Hay, David C., Winkler, David A., Barrett, David A., Davies, Martyn C., Young, Lorraine E., Denning, Chris, Alexander, Morgan R.
Format: Article
Published: Wiley 2015
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Online Access:https://eprints.nottingham.ac.uk/48601/
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author Celiz, Adam D.
Smith, James G.W.
Patel, Asha K.
Hook, Andrew L.
Rajamohan, Divya
George, Vinoj T.
Flatt, Luke
Patel, Minal J.
Epa, Vidana C.
Singh, Taranjit
Langer, Robert
Anderson, Daniel G.
Allen, Nicholas D.
Hay, David C.
Winkler, David A.
Barrett, David A.
Davies, Martyn C.
Young, Lorraine E.
Denning, Chris
Alexander, Morgan R.
author_facet Celiz, Adam D.
Smith, James G.W.
Patel, Asha K.
Hook, Andrew L.
Rajamohan, Divya
George, Vinoj T.
Flatt, Luke
Patel, Minal J.
Epa, Vidana C.
Singh, Taranjit
Langer, Robert
Anderson, Daniel G.
Allen, Nicholas D.
Hay, David C.
Winkler, David A.
Barrett, David A.
Davies, Martyn C.
Young, Lorraine E.
Denning, Chris
Alexander, Morgan R.
author_sort Celiz, Adam D.
building Nottingham Research Data Repository
collection Online Access
description A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.
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spelling nottingham-486012020-05-04T17:12:59Z https://eprints.nottingham.ac.uk/48601/ Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation Celiz, Adam D. Smith, James G.W. Patel, Asha K. Hook, Andrew L. Rajamohan, Divya George, Vinoj T. Flatt, Luke Patel, Minal J. Epa, Vidana C. Singh, Taranjit Langer, Robert Anderson, Daniel G. Allen, Nicholas D. Hay, David C. Winkler, David A. Barrett, David A. Davies, Martyn C. Young, Lorraine E. Denning, Chris Alexander, Morgan R. A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized. Wiley 2015-07-15 Article PeerReviewed Celiz, Adam D., Smith, James G.W., Patel, Asha K., Hook, Andrew L., Rajamohan, Divya, George, Vinoj T., Flatt, Luke, Patel, Minal J., Epa, Vidana C., Singh, Taranjit, Langer, Robert, Anderson, Daniel G., Allen, Nicholas D., Hay, David C., Winkler, David A., Barrett, David A., Davies, Martyn C., Young, Lorraine E., Denning, Chris and Alexander, Morgan R. (2015) Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation. Advanced Materials, 27 (27). pp. 4006-4012. ISSN 0935-9648 high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation http://onlinelibrary.wiley.com/doi/10.1002/adma.201501351/full doi:10.1002/adma.201501351 doi:10.1002/adma.201501351
spellingShingle high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation
Celiz, Adam D.
Smith, James G.W.
Patel, Asha K.
Hook, Andrew L.
Rajamohan, Divya
George, Vinoj T.
Flatt, Luke
Patel, Minal J.
Epa, Vidana C.
Singh, Taranjit
Langer, Robert
Anderson, Daniel G.
Allen, Nicholas D.
Hay, David C.
Winkler, David A.
Barrett, David A.
Davies, Martyn C.
Young, Lorraine E.
Denning, Chris
Alexander, Morgan R.
Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title_full Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title_fullStr Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title_full_unstemmed Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title_short Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
title_sort discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation
topic high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation
url https://eprints.nottingham.ac.uk/48601/
https://eprints.nottingham.ac.uk/48601/
https://eprints.nottingham.ac.uk/48601/