Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells

Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materi...

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Main Authors: Vining, Kyle H., Scherba, Jacob C., Bever, Alaina, Alexander, Morgan R., Celiz, Adam D., Mooney, David J.
Format: Article
Published: Wiley 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/48580/
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author Vining, Kyle H.
Scherba, Jacob C.
Bever, Alaina
Alexander, Morgan R.
Celiz, Adam D.
Mooney, David J.
author_facet Vining, Kyle H.
Scherba, Jacob C.
Bever, Alaina
Alexander, Morgan R.
Celiz, Adam D.
Mooney, David J.
author_sort Vining, Kyle H.
building Nottingham Research Data Repository
collection Online Access
description Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used. Based on these findings, thiol-ene chemistry is employed to achieve rapid light-curing and minimize residual monomer of the lead materials. Several triacrylate bulk polymers support DPSC adhesion, proliferation, and differentiation in vitro, and exhibit stiffness and tensile strength similar to existing dental materials. Conversely, materials composed of a trimethacrylate monomer or bisphenol A glycidyl methacrylate, which is a monomer standard in dental materials, do not support stem cell adhesion and negatively impact matrix and signaling pathways. Furthermore, thiol-ene polymerized triacrylates are used as permanent filling materials at the dentin-pulp interface in direct contact with irreversibly injured pulp tissue. These novel triacrylate-based biomaterials have potential to enable novel regenerative dental therapies in the clinic by both restoring teeth and providing a supportive niche for DPSCs.
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spelling nottingham-485802020-05-04T19:28:06Z https://eprints.nottingham.ac.uk/48580/ Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells Vining, Kyle H. Scherba, Jacob C. Bever, Alaina Alexander, Morgan R. Celiz, Adam D. Mooney, David J. Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstructive manner. There is a need for restorative materials that can support native functions of dental pulp stem cells (DPSCs), which are capable of regenerating dentin. A polymer microarray formed from commercially available monomers to rapidly identify materials that support DPSC adhesion is used. Based on these findings, thiol-ene chemistry is employed to achieve rapid light-curing and minimize residual monomer of the lead materials. Several triacrylate bulk polymers support DPSC adhesion, proliferation, and differentiation in vitro, and exhibit stiffness and tensile strength similar to existing dental materials. Conversely, materials composed of a trimethacrylate monomer or bisphenol A glycidyl methacrylate, which is a monomer standard in dental materials, do not support stem cell adhesion and negatively impact matrix and signaling pathways. Furthermore, thiol-ene polymerized triacrylates are used as permanent filling materials at the dentin-pulp interface in direct contact with irreversibly injured pulp tissue. These novel triacrylate-based biomaterials have potential to enable novel regenerative dental therapies in the clinic by both restoring teeth and providing a supportive niche for DPSCs. Wiley 2018-01-25 Article PeerReviewed Vining, Kyle H., Scherba, Jacob C., Bever, Alaina, Alexander, Morgan R., Celiz, Adam D. and Mooney, David J. (2018) Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells. Advanced Materials, 30 (4). 1704486/1-1704486/9. ISSN 1521-4095 dental materials; dental pulp stem cells; multi-functional acrylates polymer microarray; differentiation http://onlinelibrary.wiley.com/doi/10.1002/adma.201704486/abstract doi:10.1002/adma.201704486 doi:10.1002/adma.201704486
spellingShingle dental materials; dental pulp stem cells; multi-functional acrylates
polymer microarray; differentiation
Vining, Kyle H.
Scherba, Jacob C.
Bever, Alaina
Alexander, Morgan R.
Celiz, Adam D.
Mooney, David J.
Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title_full Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title_fullStr Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title_full_unstemmed Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title_short Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
title_sort synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells
topic dental materials; dental pulp stem cells; multi-functional acrylates
polymer microarray; differentiation
url https://eprints.nottingham.ac.uk/48580/
https://eprints.nottingham.ac.uk/48580/
https://eprints.nottingham.ac.uk/48580/