A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells were incubated in D2O-enriched media and myotubes ±50...

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Bibliographic Details
Main Authors: Brook, Matthew S., Wilkinson, D.J., Mitchell, W. Kyle, Lund, Jonathan N., Phillips, Bethan E., Szewczyk, Nathaniel J., Kainulainen, H., Lensu, S., Koch, L.G., Britton, S.L., Greenhaff, Paul L., Smith, K., Atherton, Philip J.
Format: Article
Published: American Physiological Society 2017
Subjects:
Ribosomal biogenesis; D2O; RNA synthesis; Muscle
Online Access:https://eprints.nottingham.ac.uk/48573/

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