Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach
Accurate quantification of the carbohydrate content of biomass is crucial for many bio-refining processes. The most commonly followed protocol is typically a modification of the NREL-based assay (specifically designed for carbohydrate analysis from lignocellulosic biomass). However, this NREL protoc...
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| Format: | Article |
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Springer Verlag
2017
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| Online Access: | https://eprints.nottingham.ac.uk/48494/ |
| _version_ | 1848797776966582272 |
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| author | Kostas, Emily Tsambika Wilkinson, Stuart White, Daniel A. Cook, David J. |
| author_facet | Kostas, Emily Tsambika Wilkinson, Stuart White, Daniel A. Cook, David J. |
| author_sort | Kostas, Emily Tsambika |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Accurate quantification of the carbohydrate content of biomass is crucial for many bio-refining processes. The most commonly followed protocol is typically a modification of the NREL-based assay (specifically designed for carbohydrate analysis from lignocellulosic biomass). However, this NREL protocol was revealed to be excessively thermochemically harsh for seaweed biomass. This can result in erroneously low total sugar quantification as the reaction severity can degrade a proportion of the liberated sugars to decomposition products such as furans. Here we describe an optimization of the total acid hydrolysis protocol for accurate quantification of the carbohydrate content of seaweeds. Different species of seaweed can be accurately evaluated for their carbohydrate contents by following this optimized method. |
| first_indexed | 2025-11-14T20:09:16Z |
| format | Article |
| id | nottingham-48494 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:09:16Z |
| publishDate | 2017 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-484942020-05-04T19:17:17Z https://eprints.nottingham.ac.uk/48494/ Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach Kostas, Emily Tsambika Wilkinson, Stuart White, Daniel A. Cook, David J. Accurate quantification of the carbohydrate content of biomass is crucial for many bio-refining processes. The most commonly followed protocol is typically a modification of the NREL-based assay (specifically designed for carbohydrate analysis from lignocellulosic biomass). However, this NREL protocol was revealed to be excessively thermochemically harsh for seaweed biomass. This can result in erroneously low total sugar quantification as the reaction severity can degrade a proportion of the liberated sugars to decomposition products such as furans. Here we describe an optimization of the total acid hydrolysis protocol for accurate quantification of the carbohydrate content of seaweeds. Different species of seaweed can be accurately evaluated for their carbohydrate contents by following this optimized method. Springer Verlag 2017-11-14 Article PeerReviewed Kostas, Emily Tsambika, Wilkinson, Stuart, White, Daniel A. and Cook, David J. (2017) Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach. Methods in Molecular Biology . ISSN 1940-6029 Carbohydrate Composition Macroalgae Mass balance Optimization Species https://link.springer.com/protocol/10.1007%2F7651_2017_105 doi:10.1007/7651_2017_105 doi:10.1007/7651_2017_105 |
| spellingShingle | Carbohydrate Composition Macroalgae Mass balance Optimization Species Kostas, Emily Tsambika Wilkinson, Stuart White, Daniel A. Cook, David J. Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title | Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title_full | Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title_fullStr | Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title_full_unstemmed | Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title_short | Complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| title_sort | complete acid-based hydrolysis assay for carbohydrate quantification in seaweed: a species-specific optimized approach |
| topic | Carbohydrate Composition Macroalgae Mass balance Optimization Species |
| url | https://eprints.nottingham.ac.uk/48494/ https://eprints.nottingham.ac.uk/48494/ https://eprints.nottingham.ac.uk/48494/ |