Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)

Background: Excessive haemorrhage at caesarean section requires donor (allogeneic) blood transfusion. Cell 34 salvage may reduce this requirement. Methods and findings: We conducted a pragmatic randomised controlled trial (26 obstetric units; June 2013 through April 2016) of routine cell salvage...

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Main Authors: Khan, Khalid S., Moore, Philip A.S., Wilson, Matthew J., Hooper, Richard, Allard, Shubha, Wrench, Ian, Beresford, Lee, Roberts, Tracy E., McLoughlin, Carol, Geoghegan, James, Daniels, Jane P., Catling, Sue, Clark, Vicki A., Ayuk, Paul, Robson, Stephen, Gao-Smith, Fang, Hogg, Matthew, Lanz, Doris, Dodds, Julie
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Published: Public Library of Science 2017
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Online Access:https://eprints.nottingham.ac.uk/48468/
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author Khan, Khalid S.
Moore, Philip A.S.
Wilson, Matthew J.
Hooper, Richard
Allard, Shubha
Wrench, Ian
Beresford, Lee
Roberts, Tracy E.
McLoughlin, Carol
Geoghegan, James
Daniels, Jane P.
Catling, Sue
Clark, Vicki A.
Ayuk, Paul
Robson, Stephen
Gao-Smith, Fang
Hogg, Matthew
Lanz, Doris
Dodds, Julie
author_facet Khan, Khalid S.
Moore, Philip A.S.
Wilson, Matthew J.
Hooper, Richard
Allard, Shubha
Wrench, Ian
Beresford, Lee
Roberts, Tracy E.
McLoughlin, Carol
Geoghegan, James
Daniels, Jane P.
Catling, Sue
Clark, Vicki A.
Ayuk, Paul
Robson, Stephen
Gao-Smith, Fang
Hogg, Matthew
Lanz, Doris
Dodds, Julie
author_sort Khan, Khalid S.
building Nottingham Research Data Repository
collection Online Access
description Background: Excessive haemorrhage at caesarean section requires donor (allogeneic) blood transfusion. Cell 34 salvage may reduce this requirement. Methods and findings: We conducted a pragmatic randomised controlled trial (26 obstetric units; June 2013 through April 2016) of routine cell salvage use (intervention) vs. current standard of care without routine salvage use (control) in caesarean section among women at risk of haemorrhage. Randomisation was stratified, using random permuted blocks of variable sizes. In an intention-to-treat analysis, we used multivariable models, adjusting for stratification variables and prognostic factors identified a priori, to compare rates of donor blood transfusion (primary outcome) and fetomaternal haemorrhage ≥2ml in RhD-negative women with RhD-positive baby (a secondary outcome) between groups. Among 3028 women randomised (2990 analysed), 95.6% of 1498 assigned to intervention had cell salvage deployed (50.8% had salvaged blood returned; mean 259.9 ml) vs. 3.9% of 1492 assigned to control. Donor blood transfusion rates were 3.5% in the control group vs. 2.5% in intervention (adjusted odds ratio [OR] 0.65, 95% confidence interval [CI] 0.42 to 1.01, p=0.056; adjusted risk difference -1.03, 95% CI -2.13 to 0.06; number needed to treat [NNT] 97, at the lower limit of 95% confidence NNT was 47 and at the upper limit the number needed to harm was 1,667). In a planned subgroup analysis, the transfusion rate was 4.6% in women assigned to control vs. 3.0% in the intervention group among emergency caesareans (adjusted OR 0.58, 95% CI 0.34 to 0.99), whereas it was 2.2% vs. 1.8% among elective caesareans (adjusted OR 0.83, 95% CI 0.38 to 1.83) (interaction p=0.46). No case of amniotic fluid embolism was observed. Fetomaternal haemorrhage was higher with intervention (10.5% in control vs. 25.6% in intervention, adjusted OR 5.63, 95% CI 1.43 to 22.14, p=0.013). We are unable to comment on long-term antibody sensitisation effects. Conclusions: The overall reduction observed in donor blood transfusion associated with the routine use of cell salvage during caesarean section was not statistically significant.
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spelling nottingham-484682020-05-04T19:17:30Z https://eprints.nottingham.ac.uk/48468/ Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO) Khan, Khalid S. Moore, Philip A.S. Wilson, Matthew J. Hooper, Richard Allard, Shubha Wrench, Ian Beresford, Lee Roberts, Tracy E. McLoughlin, Carol Geoghegan, James Daniels, Jane P. Catling, Sue Clark, Vicki A. Ayuk, Paul Robson, Stephen Gao-Smith, Fang Hogg, Matthew Lanz, Doris Dodds, Julie Background: Excessive haemorrhage at caesarean section requires donor (allogeneic) blood transfusion. Cell 34 salvage may reduce this requirement. Methods and findings: We conducted a pragmatic randomised controlled trial (26 obstetric units; June 2013 through April 2016) of routine cell salvage use (intervention) vs. current standard of care without routine salvage use (control) in caesarean section among women at risk of haemorrhage. Randomisation was stratified, using random permuted blocks of variable sizes. In an intention-to-treat analysis, we used multivariable models, adjusting for stratification variables and prognostic factors identified a priori, to compare rates of donor blood transfusion (primary outcome) and fetomaternal haemorrhage ≥2ml in RhD-negative women with RhD-positive baby (a secondary outcome) between groups. Among 3028 women randomised (2990 analysed), 95.6% of 1498 assigned to intervention had cell salvage deployed (50.8% had salvaged blood returned; mean 259.9 ml) vs. 3.9% of 1492 assigned to control. Donor blood transfusion rates were 3.5% in the control group vs. 2.5% in intervention (adjusted odds ratio [OR] 0.65, 95% confidence interval [CI] 0.42 to 1.01, p=0.056; adjusted risk difference -1.03, 95% CI -2.13 to 0.06; number needed to treat [NNT] 97, at the lower limit of 95% confidence NNT was 47 and at the upper limit the number needed to harm was 1,667). In a planned subgroup analysis, the transfusion rate was 4.6% in women assigned to control vs. 3.0% in the intervention group among emergency caesareans (adjusted OR 0.58, 95% CI 0.34 to 0.99), whereas it was 2.2% vs. 1.8% among elective caesareans (adjusted OR 0.83, 95% CI 0.38 to 1.83) (interaction p=0.46). No case of amniotic fluid embolism was observed. Fetomaternal haemorrhage was higher with intervention (10.5% in control vs. 25.6% in intervention, adjusted OR 5.63, 95% CI 1.43 to 22.14, p=0.013). We are unable to comment on long-term antibody sensitisation effects. Conclusions: The overall reduction observed in donor blood transfusion associated with the routine use of cell salvage during caesarean section was not statistically significant. Public Library of Science 2017-11-13 Article PeerReviewed Khan, Khalid S., Moore, Philip A.S., Wilson, Matthew J., Hooper, Richard, Allard, Shubha, Wrench, Ian, Beresford, Lee, Roberts, Tracy E., McLoughlin, Carol, Geoghegan, James, Daniels, Jane P., Catling, Sue, Clark, Vicki A., Ayuk, Paul, Robson, Stephen, Gao-Smith, Fang, Hogg, Matthew, Lanz, Doris and Dodds, Julie (2017) Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO). PLoS Medicine . ISSN 1549-1277 (In Press) Intraoperative Blood Cell Salvage Caesarean Section Obstetrics
spellingShingle Intraoperative Blood Cell Salvage
Caesarean Section
Obstetrics
Khan, Khalid S.
Moore, Philip A.S.
Wilson, Matthew J.
Hooper, Richard
Allard, Shubha
Wrench, Ian
Beresford, Lee
Roberts, Tracy E.
McLoughlin, Carol
Geoghegan, James
Daniels, Jane P.
Catling, Sue
Clark, Vicki A.
Ayuk, Paul
Robson, Stephen
Gao-Smith, Fang
Hogg, Matthew
Lanz, Doris
Dodds, Julie
Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title_full Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title_fullStr Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title_full_unstemmed Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title_short Cell salvage and donor blood transfusion during Caesarean section: a pragmatic multicentre randomised controlled trial (SALVO)
title_sort cell salvage and donor blood transfusion during caesarean section: a pragmatic multicentre randomised controlled trial (salvo)
topic Intraoperative Blood Cell Salvage
Caesarean Section
Obstetrics
url https://eprints.nottingham.ac.uk/48468/