RBL cells as models for in vitro studies of mast cells and basophils
Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated,have provided advantages over primary...
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| Format: | Article |
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Wiley
2018
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| Online Access: | https://eprints.nottingham.ac.uk/48455/ |
| _version_ | 1848797767401472000 |
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| author | Falcone, Franco H. Wan, Daniel Barwary, Nafal Sagi-Eisenberg, Ronit |
| author_facet | Falcone, Franco H. Wan, Daniel Barwary, Nafal Sagi-Eisenberg, Ronit |
| author_sort | Falcone, Franco H. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated,have provided advantages over primary MCs, in particular for molecular studies relying on genetic screening. Furthermore, the ability to generate clones that stably express proteins of interest, for example, a human receptor, have marked the RBL cells as an attractive MC model for drug screening. Indeed, 3 RBL reporter cell lines (RS-ATL8, NFAT-DsRed,and NPY-mRFP) have been generated providing useful models for drug and allergen screening. Similarly, RBL cells stably expressing the human MrgprX2 receptor provide a unique paradigm for analyzing ligand interactions and signaling pathways of the unique human receptor. Finally, transient co-transfections of RBL cells allow functional genomic analyses of MC secretion by combining library screening with simultaneous expression of a reporter for exocytosis. RBL cells thus comprise powerful tools for the study of intracellular membrane trafficking and exocytosis and the detection of allergens, vaccine safety studies and diagnosis of allergic sensitization. Their recent uses as an investigative tool are reviewed here. |
| first_indexed | 2025-11-14T20:09:06Z |
| format | Article |
| id | nottingham-48455 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T20:09:06Z |
| publishDate | 2018 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-484552019-02-12T04:30:10Z https://eprints.nottingham.ac.uk/48455/ RBL cells as models for in vitro studies of mast cells and basophils Falcone, Franco H. Wan, Daniel Barwary, Nafal Sagi-Eisenberg, Ronit Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically manipulated,have provided advantages over primary MCs, in particular for molecular studies relying on genetic screening. Furthermore, the ability to generate clones that stably express proteins of interest, for example, a human receptor, have marked the RBL cells as an attractive MC model for drug screening. Indeed, 3 RBL reporter cell lines (RS-ATL8, NFAT-DsRed,and NPY-mRFP) have been generated providing useful models for drug and allergen screening. Similarly, RBL cells stably expressing the human MrgprX2 receptor provide a unique paradigm for analyzing ligand interactions and signaling pathways of the unique human receptor. Finally, transient co-transfections of RBL cells allow functional genomic analyses of MC secretion by combining library screening with simultaneous expression of a reporter for exocytosis. RBL cells thus comprise powerful tools for the study of intracellular membrane trafficking and exocytosis and the detection of allergens, vaccine safety studies and diagnosis of allergic sensitization. Their recent uses as an investigative tool are reviewed here. Wiley 2018-03-30 Article PeerReviewed application/pdf en https://eprints.nottingham.ac.uk/48455/8/Final%20Immunolo%20Review%2026-10-17RSE.pdf application/pdf en https://eprints.nottingham.ac.uk/48455/1/imr_12628_Rev%20Final.pdf Falcone, Franco H., Wan, Daniel, Barwary, Nafal and Sagi-Eisenberg, Ronit (2018) RBL cells as models for in vitro studies of mast cells and basophils. Immunological Reviews, 282 (1). pp. 47-57. ISSN 1600-065X in vitro mast cell model NFAT-DsRed NPY-mRFP RBL-2H3 RS-ATL8 http://onlinelibrary.wiley.com/doi/10.1111/imr.12628/abstract doi:10.1111/imr.12628 doi:10.1111/imr.12628 |
| spellingShingle | in vitro mast cell model NFAT-DsRed NPY-mRFP RBL-2H3 RS-ATL8 Falcone, Franco H. Wan, Daniel Barwary, Nafal Sagi-Eisenberg, Ronit RBL cells as models for in vitro studies of mast cells and basophils |
| title | RBL cells as models for in vitro studies of mast cells and basophils |
| title_full | RBL cells as models for in vitro studies of mast cells and basophils |
| title_fullStr | RBL cells as models for in vitro studies of mast cells and basophils |
| title_full_unstemmed | RBL cells as models for in vitro studies of mast cells and basophils |
| title_short | RBL cells as models for in vitro studies of mast cells and basophils |
| title_sort | rbl cells as models for in vitro studies of mast cells and basophils |
| topic | in vitro mast cell model NFAT-DsRed NPY-mRFP RBL-2H3 RS-ATL8 |
| url | https://eprints.nottingham.ac.uk/48455/ https://eprints.nottingham.ac.uk/48455/ https://eprints.nottingham.ac.uk/48455/ |