Efficient bio-production of citramalate using an engineered Escherichia coli strain
Citramalic acid is a central intermediate in a combined biocatalytic and chemocatalytic route to produce bio-based methylmethacrylate, the monomer used to manufacture Perspex and other high performance materials. We developed an engineered E. coli strain and a fed-batch bioprocess to produce citrama...
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Society for General Microbiology
2018
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| Online Access: | https://eprints.nottingham.ac.uk/48425/ |
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| author | Webb, Joseph P. Arnold, S. Alison Baxter, Scott Hall, Stephen J. Eastham, Graham Stephens, Gill |
| author_facet | Webb, Joseph P. Arnold, S. Alison Baxter, Scott Hall, Stephen J. Eastham, Graham Stephens, Gill |
| author_sort | Webb, Joseph P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Citramalic acid is a central intermediate in a combined biocatalytic and chemocatalytic route to produce bio-based methylmethacrylate, the monomer used to manufacture Perspex and other high performance materials. We developed an engineered E. coli strain and a fed-batch bioprocess to produce citramalate at concentrations in excess of 80 g l-1 in only 65 h. This exceptional efficiency was achieved by designing the production strain and the fermentation system to operate synergistically. Thus, a single gene encoding a mesophilic variant of citramalate synthase from Methanococcus jannaschii, CimA3.7, was expressed in E. coli to convert acetyl-CoA and pyruvate to citramalate, and the ldhA and pflB genes were deleted. By using a bioprocess with a continuous, growth-limiting feed of glucose, these simple interventions diverted substrate flux directly from central metabolism towards formation of citramalate, without problematic accumulation of acetate. Furthermore, the nutritional requirements of the production strain could be satisfied through the use of a mineral salts medium supplemented only with glucose (172 g l-1 in total) and 1.4 g l-1 yeast extract. Using this system, citramalate accumulated to 82±1.5 g l-1, with a productivity of 1.85 g l-1 h-1 and a conversion efficiency of 0.48 gcitramalate g-1 glucose. The new bioprocess forms a practical first step for integrated bio- and chemocatalytic production of methylmethacrylate. |
| first_indexed | 2025-11-14T20:09:00Z |
| format | Article |
| id | nottingham-48425 |
| institution | University of Nottingham Malaysia Campus |
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| last_indexed | 2025-11-14T20:09:00Z |
| publishDate | 2018 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
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| spelling | nottingham-484252020-05-04T19:30:35Z https://eprints.nottingham.ac.uk/48425/ Efficient bio-production of citramalate using an engineered Escherichia coli strain Webb, Joseph P. Arnold, S. Alison Baxter, Scott Hall, Stephen J. Eastham, Graham Stephens, Gill Citramalic acid is a central intermediate in a combined biocatalytic and chemocatalytic route to produce bio-based methylmethacrylate, the monomer used to manufacture Perspex and other high performance materials. We developed an engineered E. coli strain and a fed-batch bioprocess to produce citramalate at concentrations in excess of 80 g l-1 in only 65 h. This exceptional efficiency was achieved by designing the production strain and the fermentation system to operate synergistically. Thus, a single gene encoding a mesophilic variant of citramalate synthase from Methanococcus jannaschii, CimA3.7, was expressed in E. coli to convert acetyl-CoA and pyruvate to citramalate, and the ldhA and pflB genes were deleted. By using a bioprocess with a continuous, growth-limiting feed of glucose, these simple interventions diverted substrate flux directly from central metabolism towards formation of citramalate, without problematic accumulation of acetate. Furthermore, the nutritional requirements of the production strain could be satisfied through the use of a mineral salts medium supplemented only with glucose (172 g l-1 in total) and 1.4 g l-1 yeast extract. Using this system, citramalate accumulated to 82±1.5 g l-1, with a productivity of 1.85 g l-1 h-1 and a conversion efficiency of 0.48 gcitramalate g-1 glucose. The new bioprocess forms a practical first step for integrated bio- and chemocatalytic production of methylmethacrylate. Society for General Microbiology 2018-02-01 Article PeerReviewed Webb, Joseph P., Arnold, S. Alison, Baxter, Scott, Hall, Stephen J., Eastham, Graham and Stephens, Gill (2018) Efficient bio-production of citramalate using an engineered Escherichia coli strain. Microbiology, 164 . pp. 133-141. ISSN 1465-2080 citramalic acid; methyl methacrylate; fed-batch fermentation; bio-based chemicals http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000581 doi:10.1099/mic.0.000581 doi:10.1099/mic.0.000581 |
| spellingShingle | citramalic acid; methyl methacrylate; fed-batch fermentation; bio-based chemicals Webb, Joseph P. Arnold, S. Alison Baxter, Scott Hall, Stephen J. Eastham, Graham Stephens, Gill Efficient bio-production of citramalate using an engineered Escherichia coli strain |
| title | Efficient bio-production of citramalate using an engineered
Escherichia coli strain |
| title_full | Efficient bio-production of citramalate using an engineered
Escherichia coli strain |
| title_fullStr | Efficient bio-production of citramalate using an engineered
Escherichia coli strain |
| title_full_unstemmed | Efficient bio-production of citramalate using an engineered
Escherichia coli strain |
| title_short | Efficient bio-production of citramalate using an engineered
Escherichia coli strain |
| title_sort | efficient bio-production of citramalate using an engineered
escherichia coli strain |
| topic | citramalic acid; methyl methacrylate; fed-batch fermentation; bio-based chemicals |
| url | https://eprints.nottingham.ac.uk/48425/ https://eprints.nottingham.ac.uk/48425/ https://eprints.nottingham.ac.uk/48425/ |