Long-term CO₂ injection and its impact on near-surface soil microbiology

Impacts of long-term CO₂ exposure on environmental processes and microbial populations of near-surface soils are poorly understood. This near-surface long-term CO₂ injection study demonstrated that soil microbiology and geochemistry is influenced more by seasonal parameters than elevated CO₂. Soil s...

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Main Authors: Gwosdz, Simone, West, Julia M., Jones, David, Rakoczy, Jana, Green, Kay, Barlow, Tom, Blöthe, Marco, Smith, Karon L., Steven, Michael D., Krüger, Martin
Format: Article
Published: Oxford University Press 2016
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Online Access:https://eprints.nottingham.ac.uk/47911/
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author Gwosdz, Simone
West, Julia M.
Jones, David
Rakoczy, Jana
Green, Kay
Barlow, Tom
Blöthe, Marco
Smith, Karon L.
Steven, Michael D.
Krüger, Martin
author_facet Gwosdz, Simone
West, Julia M.
Jones, David
Rakoczy, Jana
Green, Kay
Barlow, Tom
Blöthe, Marco
Smith, Karon L.
Steven, Michael D.
Krüger, Martin
author_sort Gwosdz, Simone
building Nottingham Research Data Repository
collection Online Access
description Impacts of long-term CO₂ exposure on environmental processes and microbial populations of near-surface soils are poorly understood. This near-surface long-term CO₂ injection study demonstrated that soil microbiology and geochemistry is influenced more by seasonal parameters than elevated CO₂. Soil samples were taken during a 3-year field experiment including sampling campaigns before, during and after 24 months of continuous CO₂ injection. CO₂ concentrations within CO₂-injected plots increased up to 23% during the injection period. No CO₂ impacts on geochemistry were detected over time. In addition, CO₂ exposed samples did not show significant changes in microbial CO₂ and CH₄ turnover rates compared to reference samples. Likewise, no significant CO₂-induced variations were detected for the abundance of Bacteria, Archaea (16S rDNA) and gene copy numbers of the mcrA gene, Crenarchaeota and amoA gene. The majority (75%–95%) of the bacterial sequences were assigned to five phyla: Firmicutes, Proteobacteria, Actinobacteria, Acidobacteria and Bacteroidetes. The majority of the archaeal sequences (85%–100%) were assigned to the thaumarchaeotal cluster I.1b (soil group). Univariate and multivariate statistical as well as principal component analyses showed no significant CO₂-induced variation. Instead, seasonal impacts especially temperature and precipitation were detected.
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spelling nottingham-479112020-05-04T18:18:40Z https://eprints.nottingham.ac.uk/47911/ Long-term CO₂ injection and its impact on near-surface soil microbiology Gwosdz, Simone West, Julia M. Jones, David Rakoczy, Jana Green, Kay Barlow, Tom Blöthe, Marco Smith, Karon L. Steven, Michael D. Krüger, Martin Impacts of long-term CO₂ exposure on environmental processes and microbial populations of near-surface soils are poorly understood. This near-surface long-term CO₂ injection study demonstrated that soil microbiology and geochemistry is influenced more by seasonal parameters than elevated CO₂. Soil samples were taken during a 3-year field experiment including sampling campaigns before, during and after 24 months of continuous CO₂ injection. CO₂ concentrations within CO₂-injected plots increased up to 23% during the injection period. No CO₂ impacts on geochemistry were detected over time. In addition, CO₂ exposed samples did not show significant changes in microbial CO₂ and CH₄ turnover rates compared to reference samples. Likewise, no significant CO₂-induced variations were detected for the abundance of Bacteria, Archaea (16S rDNA) and gene copy numbers of the mcrA gene, Crenarchaeota and amoA gene. The majority (75%–95%) of the bacterial sequences were assigned to five phyla: Firmicutes, Proteobacteria, Actinobacteria, Acidobacteria and Bacteroidetes. The majority of the archaeal sequences (85%–100%) were assigned to the thaumarchaeotal cluster I.1b (soil group). Univariate and multivariate statistical as well as principal component analyses showed no significant CO₂-induced variation. Instead, seasonal impacts especially temperature and precipitation were detected. Oxford University Press 2016-12-01 Article PeerReviewed Gwosdz, Simone, West, Julia M., Jones, David, Rakoczy, Jana, Green, Kay, Barlow, Tom, Blöthe, Marco, Smith, Karon L., Steven, Michael D. and Krüger, Martin (2016) Long-term CO₂ injection and its impact on near-surface soil microbiology. FEMS Microbiology Ecology, 92 (12). fiw193/1-fiw193/10. ISSN 1574-6941 CCS; CO₂-leakage; Bacteria; Archaea; qPCR; pyrosequencing https://academic.oup.com/femsec/article/92/12/fiw193/2570443 doi:10.1093/femsec/fiw193 doi:10.1093/femsec/fiw193
spellingShingle CCS; CO₂-leakage; Bacteria; Archaea; qPCR; pyrosequencing
Gwosdz, Simone
West, Julia M.
Jones, David
Rakoczy, Jana
Green, Kay
Barlow, Tom
Blöthe, Marco
Smith, Karon L.
Steven, Michael D.
Krüger, Martin
Long-term CO₂ injection and its impact on near-surface soil microbiology
title Long-term CO₂ injection and its impact on near-surface soil microbiology
title_full Long-term CO₂ injection and its impact on near-surface soil microbiology
title_fullStr Long-term CO₂ injection and its impact on near-surface soil microbiology
title_full_unstemmed Long-term CO₂ injection and its impact on near-surface soil microbiology
title_short Long-term CO₂ injection and its impact on near-surface soil microbiology
title_sort long-term co₂ injection and its impact on near-surface soil microbiology
topic CCS; CO₂-leakage; Bacteria; Archaea; qPCR; pyrosequencing
url https://eprints.nottingham.ac.uk/47911/
https://eprints.nottingham.ac.uk/47911/
https://eprints.nottingham.ac.uk/47911/