Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics
Calcium (Ca) and magnesium (Mg) are essential plant nutrients and vital for human and animal nutrition. Biofortification of crops has previously been suggested to alleviate widespread human Ca and Mg deficiencies. In this study, new candidate genes influencing the leaf accumulation of Ca and Mg were...
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Frontiers
2017
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| Online Access: | https://eprints.nottingham.ac.uk/47818/ |
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| author | Alcock, T.D. Havlickova, Lenka He, Zhesi Bancroft, I. White, Philip J. Broadley, Martin R. Graham, Neil S. |
| author_facet | Alcock, T.D. Havlickova, Lenka He, Zhesi Bancroft, I. White, Philip J. Broadley, Martin R. Graham, Neil S. |
| author_sort | Alcock, T.D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Calcium (Ca) and magnesium (Mg) are essential plant nutrients and vital for human and animal nutrition. Biofortification of crops has previously been suggested to alleviate widespread human Ca and Mg deficiencies. In this study, new candidate genes influencing the leaf accumulation of Ca and Mg were identified in young Brassica napus plants using associative transcriptomics of bionomics datasets. A total of 247 and 166 SNP markers were associated with leaf Ca and Mg concentration, respectively, after false discovery rate correction and removal of SNPs with low second allele frequency. Gene expression markers at similar positions were also associated with leaf Ca and Mg concentration, including loci on chromosomes A10 and C2, within which lie previously identified transporter genes ACA8 and MGT7. Further candidate genes were selected from seven loci and the mineral composition of whole Arabidopsis thaliana shoots were characterized from lines mutated in orthologous genes. Four and two mutant lines had reduced shoot Ca and Mg concentration, respectively, compared to wild type plants. Three of these mutations were found to have tissue specific effects; notably reduced silique Ca in all three such mutant lines. This knowledge could be applied in targeted breeding, with the possibility of increasing Ca and Mg in plant tissue for improving human and livestock nutrition. |
| first_indexed | 2025-11-14T20:07:02Z |
| format | Article |
| id | nottingham-47818 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:07:02Z |
| publishDate | 2017 |
| publisher | Frontiers |
| recordtype | eprints |
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| spelling | nottingham-478182020-05-04T19:17:49Z https://eprints.nottingham.ac.uk/47818/ Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics Alcock, T.D. Havlickova, Lenka He, Zhesi Bancroft, I. White, Philip J. Broadley, Martin R. Graham, Neil S. Calcium (Ca) and magnesium (Mg) are essential plant nutrients and vital for human and animal nutrition. Biofortification of crops has previously been suggested to alleviate widespread human Ca and Mg deficiencies. In this study, new candidate genes influencing the leaf accumulation of Ca and Mg were identified in young Brassica napus plants using associative transcriptomics of bionomics datasets. A total of 247 and 166 SNP markers were associated with leaf Ca and Mg concentration, respectively, after false discovery rate correction and removal of SNPs with low second allele frequency. Gene expression markers at similar positions were also associated with leaf Ca and Mg concentration, including loci on chromosomes A10 and C2, within which lie previously identified transporter genes ACA8 and MGT7. Further candidate genes were selected from seven loci and the mineral composition of whole Arabidopsis thaliana shoots were characterized from lines mutated in orthologous genes. Four and two mutant lines had reduced shoot Ca and Mg concentration, respectively, compared to wild type plants. Three of these mutations were found to have tissue specific effects; notably reduced silique Ca in all three such mutant lines. This knowledge could be applied in targeted breeding, with the possibility of increasing Ca and Mg in plant tissue for improving human and livestock nutrition. Frontiers 2017-11-15 Article PeerReviewed Alcock, T.D., Havlickova, Lenka, He, Zhesi, Bancroft, I., White, Philip J., Broadley, Martin R. and Graham, Neil S. (2017) Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics. Frontiers in Plant Science, 8 . 2081/1-2081/13. ISSN 1664-462X Associative transcriptomics; GWAS; Brassica napus; Calcium; Magnesium; Biofortification; Nutrient use efficiency https://www.frontiersin.org/articles/10.3389/fpls.2017.01968/full doi:10.3389/fpls.2017.01968 doi:10.3389/fpls.2017.01968 |
| spellingShingle | Associative transcriptomics; GWAS; Brassica napus; Calcium; Magnesium; Biofortification; Nutrient use efficiency Alcock, T.D. Havlickova, Lenka He, Zhesi Bancroft, I. White, Philip J. Broadley, Martin R. Graham, Neil S. Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title | Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title_full | Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title_fullStr | Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title_full_unstemmed | Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title_short | Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics |
| title_sort | identification of candidate genes for calcium and magnesium accumulation in brassica napus l. by association genetics |
| topic | Associative transcriptomics; GWAS; Brassica napus; Calcium; Magnesium; Biofortification; Nutrient use efficiency |
| url | https://eprints.nottingham.ac.uk/47818/ https://eprints.nottingham.ac.uk/47818/ https://eprints.nottingham.ac.uk/47818/ |