Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

QR Code

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants

Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostri...

Full description

Bibliographic Details
Main Authors:	Grosse-Honebrink, Alexander, Schwarz, Katrin, Wang, Hengzheng, Minton, Nigel P., Zhang, Ying
Format:	Article
Published:	Elsevier 2017
Subjects:	Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC)
Online Access:	https://eprints.nottingham.ac.uk/47404/

Similar Items

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum
by: Schwarz, Katrin, et al.
Published: (2017)

Forward and reverse genetics in industrially important Clostridia
by: Grosse-Honebrink, Alexander
Published: (2017)

Synthetic biology & metabolic engineering of Clostridium pasteurianum
by: Ortega, David
Published: (2020)

Exposing hidden putative lipoproteins in Clostridium difficile
by: Griffin, R., et al.
Published: (2017)

Both, toxin A and toxin B, are important in Clostridium difficile infection
by: Kuehne, Sarah A., et al.
Published: (2011)

Towards safe and stable Clostridium botulinum strains for research and industrial applications
by: Groothuis, Daphne
Published: (2022)

Characterisation of a putative agr system in Clostridium botulinum and Clostridium sporogenes
by: Cooksley, Clare Marie
Published: (2008)

Effects of Bifidobacterium Pseudocatenulatum G4 on Clostridium Scindens and Clostridium Hiranonis
by: Rasti, Babak
Published: (2009)

A genetic assay for gene essentiality in Clostridium
by: Walker, David J.F., et al.
Published: (2016)

Development of an inducible transposon system for efficient random mutagenesis in Clostridium acetobutylicum
by: Zhang, Ying, et al.
Published: (2016)

Identification and characterisation of tail components modulating host range of Clostridium difficile phages
by: Steczynska, Joanna
Published: (2023)

The repair of DNA breaks in Escherichia coli and analysis of the bacterial recombination protein RecN
by: Wood, Stuart Robert
Published: (2009)

Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile
by: van Eijk, Erika, et al.
Published: (2016)

Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile
by: van Eijk, Erika, et al.
Published: (2016)

Amino acids utilisation by Clostridium difficile strains
by: Ogbu, H.I.
Published: (2016)

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
by: Willson, Benjamin J., et al.
Published: (2016)

Implementation and optimisation of alternative therapeutics for use in Clostridium sporogenes as a delivery vehicle
by: Budd, Patrick G.
Published: (2017)

Towards development of a liposome-based vaccine to combat Clostridium difficile infection
by: Palazi, Panayiota
Published: (2021)

The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools
by: Little, Gareth T., et al.
Published: (2018)

Profiling humoral immune responses to Clostridium difficile-specific antigens by protein microarray analysis
by: Negm, Ola H., et al.
Published: (2015)

Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain
by: Kuehne, Sarah A., et al.
Published: (2013)

Regulation of human heterochromatin protein 1 binding protein 3 (HP1BP3, also known as HP1BP74) in response to the DNA damage
by: Normatova, Makhliyo
Published: (2016)

Bioelectricity generation from sago hampas by Clostridium beijerinckii SR1 using microbial fuel cells
by: Jenol, Mohd Azwan
Published: (2020)

Using high-throughput approaches to unveil sporulation and germination mechanisms in Clostridium sporogenes
by: Mesquita Rodrigues, Raquel
Published: (2020)

Flow characteristics of carbon fibre moulding compounds
by: Evans, A.D., et al.
Published: (2016)

Exploiting the optical reflectance behaviour of carbon fibre composites for low-cost inspection and orientations analysis
by: Pierce, Robert S, et al.
Published: (2020)

The Genetic Domestication of Clostridium carboxidivorans
by: Morris, William
Published: (2025)

Analysis of the spore germination mechanisms of Clostridium difficile
by: Burns, David Alexander
Published: (2011)

Protective antibodies against Clostridium difficile are present in intravenous immunoglobulin and are retained in humans following its administration
by: Negm, Ola H., et al.
Published: (2017)

Morphological and Genetic Variation of Three-spined Stickleback (Gasterosteus aculeatus) in a Hybrid Zone
by: Bodkin, Harry
Published: (2025)

A roadmap for gene system development in Clostridium
by: Minton, Nigel P., et al.
Published: (2016)

Identification and characterisation of potential bacteriocins with biological activity against Clostridium difficile from environmental samples
by: Kim, Sungchan
Published: (2018)

Metabolic engineering of Clostridium autoethanogenum for selective alcohol production
by: Liew, Fungmin, et al.
Published: (2017)

Simultaneous saccharification and fermentation of sago hampas into biobutanol by Clostridium acetobutylicum ATCC 824
by: Husin, Hazwani
Published: (2018)

Genome-scale metabolic modelling of Clostridium autoethanogenum
by: Norman, Rupert O.J.
Published: (2019)

Role of RNPP and Agr quorum sensing in the regulation of stationary phase phenotypes in Clostridium acetobutylicum
by: Bean, Zak
Published: (2017)

Obtaining a lytic bacteriophage suitable for use in phage therapy against Clostridioides difficile infection
by: Kerr, Sarah
Published: (2023)

Biohydrogen production from sago hampas by Clostridium butyricum A1
by: Jenol, Mohd Azwan
Published: (2014)

Maternal toxicity and teratogenic effects of Jatropha curcas L. crude oil in pregnant Sprague dawley rats
by: Samat, Yon Thannia
Published: (2013)

Influence of benzene and genetic polymorphisms towards internal metabolite changes and chromosomal damage among traffic policemen in the Klang Valley, Malaysia (RESTRICTED)
by: Noor Fatihah, Mohamad Fandi
Published: (2020)