Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostri...
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/47404/ |
| _version_ | 1848797537594507264 |
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| author | Grosse-Honebrink, Alexander Schwarz, Katrin Wang, Hengzheng Minton, Nigel P. Zhang, Ying |
| author_facet | Grosse-Honebrink, Alexander Schwarz, Katrin Wang, Hengzheng Minton, Nigel P. Zhang, Ying |
| author_sort | Grosse-Honebrink, Alexander |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude. |
| first_indexed | 2025-11-14T20:05:27Z |
| format | Article |
| id | nottingham-47404 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:05:27Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-474042020-05-04T19:24:58Z https://eprints.nottingham.ac.uk/47404/ Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants Grosse-Honebrink, Alexander Schwarz, Katrin Wang, Hengzheng Minton, Nigel P. Zhang, Ying Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostridium pasteurianum by three to four orders of magnitude. Elsevier 2017-12-31 Article PeerReviewed Grosse-Honebrink, Alexander, Schwarz, Katrin, Wang, Hengzheng, Minton, Nigel P. and Zhang, Ying (2017) Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants. Anaerobe, 48 . pp. 203-205. ISSN 1095-8274 Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC) https://doi.org/10.1016/j.anaerobe.2017.09.001 doi:10.1016/j.anaerobe.2017.09.001 doi:10.1016/j.anaerobe.2017.09.001 |
| spellingShingle | Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC) Grosse-Honebrink, Alexander Schwarz, Katrin Wang, Hengzheng Minton, Nigel P. Zhang, Ying Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title | Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title_full | Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title_fullStr | Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title_full_unstemmed | Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title_short | Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants |
| title_sort | improving gene transfer in clostridium pasteurianum through the isolation of rare hypertransformable variants |
| topic | Clostridium pasteurianum; DNA transfer/transformation efficiency; Structural maintenance of chromosomes (SMC) |
| url | https://eprints.nottingham.ac.uk/47404/ https://eprints.nottingham.ac.uk/47404/ https://eprints.nottingham.ac.uk/47404/ |