Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity
Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes re...
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/47337/ |
| _version_ | 1848797520386326528 |
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| author | Pickles, S.F. Pritchard, David I. |
| author_facet | Pickles, S.F. Pritchard, David I. |
| author_sort | Pickles, S.F. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay. |
| first_indexed | 2025-11-14T20:05:11Z |
| format | Article |
| id | nottingham-47337 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:05:11Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-473372020-05-04T18:43:12Z https://eprints.nottingham.ac.uk/47337/ Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity Pickles, S.F. Pritchard, David I. Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay. Wiley 2017-04-25 Article NonPeerReviewed Pickles, S.F. and Pritchard, David I. (2017) Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Medical and Veterinary Entomology, 31 (2). pp. 200-206. ISSN 1365-2915 Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract doi:10.1111/mve.12220 doi:10.1111/mve.12220 |
| spellingShingle | Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management Pickles, S.F. Pritchard, David I. Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title | Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title_full | Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title_fullStr | Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title_full_unstemmed | Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title_short | Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity |
| title_sort | quality control of a medicinal larval (lucilia sericata) debridement device based on released gelatinase activity |
| topic | Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management |
| url | https://eprints.nottingham.ac.uk/47337/ https://eprints.nottingham.ac.uk/47337/ https://eprints.nottingham.ac.uk/47337/ |