Luminance adaptive biomarker detection in digital pathology images

Digital pathology is set to revolutionise traditional approaches diagnosing and researching diseases. To realise the full potential of digital pathology, accurate and robust computer techniques for automatically detecting biomarkers play an important role. Traditional methods transform the colour hi...

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Main Authors: Liu, Jingxin, Qiu, Guoping, Shen, Linlin
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/47281/
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author Liu, Jingxin
Qiu, Guoping
Shen, Linlin
author_facet Liu, Jingxin
Qiu, Guoping
Shen, Linlin
author_sort Liu, Jingxin
building Nottingham Research Data Repository
collection Online Access
description Digital pathology is set to revolutionise traditional approaches diagnosing and researching diseases. To realise the full potential of digital pathology, accurate and robust computer techniques for automatically detecting biomarkers play an important role. Traditional methods transform the colour histopathology images into a gray scale image and apply a single threshold to separate positively stained tissues from the background. In this paper, we show that the colour distribution of the positive immunohis-tochemical stains varies with the level of luminance and that a single threshold will be impossible to separate positively stained tissues from other tissues, regardless how the colour pixels are transformed. Based on this, we propose two novel luminance adaptive biomarker detection methods. We present experimental results to show that the luminance adaptive approach significantly improves biomarker detection accuracy and that random forest based techniques have the best performances.
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institution University of Nottingham Malaysia Campus
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publishDate 2016
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spelling nottingham-472812020-05-04T17:59:49Z https://eprints.nottingham.ac.uk/47281/ Luminance adaptive biomarker detection in digital pathology images Liu, Jingxin Qiu, Guoping Shen, Linlin Digital pathology is set to revolutionise traditional approaches diagnosing and researching diseases. To realise the full potential of digital pathology, accurate and robust computer techniques for automatically detecting biomarkers play an important role. Traditional methods transform the colour histopathology images into a gray scale image and apply a single threshold to separate positively stained tissues from the background. In this paper, we show that the colour distribution of the positive immunohis-tochemical stains varies with the level of luminance and that a single threshold will be impossible to separate positively stained tissues from other tissues, regardless how the colour pixels are transformed. Based on this, we propose two novel luminance adaptive biomarker detection methods. We present experimental results to show that the luminance adaptive approach significantly improves biomarker detection accuracy and that random forest based techniques have the best performances. Elsevier 2016-07-25 Article PeerReviewed Liu, Jingxin, Qiu, Guoping and Shen, Linlin (2016) Luminance adaptive biomarker detection in digital pathology images. Procedia Computer Science, 90 . pp. 113-118. ISSN 18770509 Immunohistochemistry; diaminobenzidine; image analysis; luminance; Random Forest http://www.sciencedirect.com/science/article/pii/S1877050916312121?via%3Dihub doi:10.1016/j.procs.2016.07.032 doi:10.1016/j.procs.2016.07.032
spellingShingle Immunohistochemistry; diaminobenzidine; image analysis; luminance; Random Forest
Liu, Jingxin
Qiu, Guoping
Shen, Linlin
Luminance adaptive biomarker detection in digital pathology images
title Luminance adaptive biomarker detection in digital pathology images
title_full Luminance adaptive biomarker detection in digital pathology images
title_fullStr Luminance adaptive biomarker detection in digital pathology images
title_full_unstemmed Luminance adaptive biomarker detection in digital pathology images
title_short Luminance adaptive biomarker detection in digital pathology images
title_sort luminance adaptive biomarker detection in digital pathology images
topic Immunohistochemistry; diaminobenzidine; image analysis; luminance; Random Forest
url https://eprints.nottingham.ac.uk/47281/
https://eprints.nottingham.ac.uk/47281/
https://eprints.nottingham.ac.uk/47281/