Embryo cell allocation patterns are not altered by biopsy but can be linked with further development
It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2 cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo b...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
BioScientifica
2017
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/47235/ |
| _version_ | 1848797496447336448 |
|---|---|
| author | Sepulveda-Rincon, Lessly P. Islam, Nadia Marsters, Peter Campbell, Bruce K. Beaujean, Nathalie Maalouf, Walid E. |
| author_facet | Sepulveda-Rincon, Lessly P. Islam, Nadia Marsters, Peter Campbell, Bruce K. Beaujean, Nathalie Maalouf, Walid E. |
| author_sort | Sepulveda-Rincon, Lessly P. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2 cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo biopsy on cell allocation patterns during embryo pre-implantation in two different mouse strains and the effects of these patterns on further development. First, one blastomere of the 2-cell embryo was injected with a lipophilic tracer and cell allocation patterns were observed at blastocyst stage. Blastocysts were classified into orthogonal, deviant or random pattern. For the first experiment, embryos were biopsied at 8-cell stage and total cell counts (TCC) were annotated. Furthermore, non biopsied blastocysts were transferred into foster mothers. Then, pups and their organs were weighed two weeks after birth. Random pattern was significantly recurrent (≈60%), against orthogonal (<22%) and deviant (<22%) patterns among groups. These patterns were not affected by biopsy procedure. However, TCC on deviant embryos were reduced after biopsy. Moreover, no differences were found between patterns for implantation rates, litter size, live offspring and organ weights (lungs, liver, pancreas and spleen). However, deviant pups presented heavier hearts and orthogonal pups presented lighter kidneys among the group. In conclusion, these results suggest that single blastomere removal does not disturb cell allocation patterns during pre-implantation. Nonetheless, the results suggest that embryos following different cell allocation patterns present different coping mechanisms against in-vitro manipulations and further development might be altered. |
| first_indexed | 2025-11-14T20:04:48Z |
| format | Article |
| id | nottingham-47235 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:04:48Z |
| publishDate | 2017 |
| publisher | BioScientifica |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-472352024-08-15T15:24:11Z https://eprints.nottingham.ac.uk/47235/ Embryo cell allocation patterns are not altered by biopsy but can be linked with further development Sepulveda-Rincon, Lessly P. Islam, Nadia Marsters, Peter Campbell, Bruce K. Beaujean, Nathalie Maalouf, Walid E. It has been suggested that first embryo cleavage can be related with the embryonic-abembryonic axis at blastocyst stage in mice. Thus, cells of the 2 cell embryo might be already biased to form the inner cell mass or trophectoderm. This study was conducted to observe the possible effects of embryo biopsy on cell allocation patterns during embryo pre-implantation in two different mouse strains and the effects of these patterns on further development. First, one blastomere of the 2-cell embryo was injected with a lipophilic tracer and cell allocation patterns were observed at blastocyst stage. Blastocysts were classified into orthogonal, deviant or random pattern. For the first experiment, embryos were biopsied at 8-cell stage and total cell counts (TCC) were annotated. Furthermore, non biopsied blastocysts were transferred into foster mothers. Then, pups and their organs were weighed two weeks after birth. Random pattern was significantly recurrent (≈60%), against orthogonal (<22%) and deviant (<22%) patterns among groups. These patterns were not affected by biopsy procedure. However, TCC on deviant embryos were reduced after biopsy. Moreover, no differences were found between patterns for implantation rates, litter size, live offspring and organ weights (lungs, liver, pancreas and spleen). However, deviant pups presented heavier hearts and orthogonal pups presented lighter kidneys among the group. In conclusion, these results suggest that single blastomere removal does not disturb cell allocation patterns during pre-implantation. Nonetheless, the results suggest that embryos following different cell allocation patterns present different coping mechanisms against in-vitro manipulations and further development might be altered. BioScientifica 2017-09-29 Article PeerReviewed Sepulveda-Rincon, Lessly P., Islam, Nadia, Marsters, Peter, Campbell, Bruce K., Beaujean, Nathalie and Maalouf, Walid E. (2017) Embryo cell allocation patterns are not altered by biopsy but can be linked with further development. Reproduction . ISSN 1470-1626 Embryo biopsy pre-patterning PGD/PGS organ development embryo transfer implantation. http://www.reproduction-online.org/content/early/2017/09/29/REP-17-0514 doi:10.1530/REP-17-0514 doi:10.1530/REP-17-0514 |
| spellingShingle | Embryo biopsy pre-patterning PGD/PGS organ development embryo transfer implantation. Sepulveda-Rincon, Lessly P. Islam, Nadia Marsters, Peter Campbell, Bruce K. Beaujean, Nathalie Maalouf, Walid E. Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title | Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title_full | Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title_fullStr | Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title_full_unstemmed | Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title_short | Embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| title_sort | embryo cell allocation patterns are not altered by biopsy but can be linked with further development |
| topic | Embryo biopsy pre-patterning PGD/PGS organ development embryo transfer implantation. |
| url | https://eprints.nottingham.ac.uk/47235/ https://eprints.nottingham.ac.uk/47235/ https://eprints.nottingham.ac.uk/47235/ |