Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports

In this clinical study, for the first time we used the direct gene therapy to restore severe injuries of the suspensory ligament branch and superficial digital flexor tendon in horses (Equus caballus). We injected the plasmid DNA encoding two therapeutic species-specific growth factors: vascular end...

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Main Authors: Kovac, Milomir, Litvin, Yaroslav A., Aliev, Ruslan O., Zakirova, Elena Yu, Rutland, Catrin S., Kiyasov, Andrey, Rizvanov, Albert A.
Format: Article
Published: Frontiers 2017
Subjects:
Online Access:https://eprints.nottingham.ac.uk/47109/
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author Kovac, Milomir
Litvin, Yaroslav A.
Aliev, Ruslan O.
Zakirova, Elena Yu
Rutland, Catrin S.
Kiyasov, Andrey
Rizvanov, Albert A.
author_facet Kovac, Milomir
Litvin, Yaroslav A.
Aliev, Ruslan O.
Zakirova, Elena Yu
Rutland, Catrin S.
Kiyasov, Andrey
Rizvanov, Albert A.
author_sort Kovac, Milomir
building Nottingham Research Data Repository
collection Online Access
description In this clinical study, for the first time we used the direct gene therapy to restore severe injuries of the suspensory ligament branch and superficial digital flexor tendon in horses (Equus caballus). We injected the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor 164 and fibroblast growth factor 2 at the site of injury in the suspensory ligament branch and tendon. Treatment effects were evaluated with the use of clinical observation and ultrasound imaging during a period of a few months. We showed that gene therapy used within a period of 2–3 months after the injury resulted in the complete recovery of functions and full restoration of the severely damaged suspensory ligament and superficial digital flexor tendon.
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spelling nottingham-471092020-05-04T19:12:11Z https://eprints.nottingham.ac.uk/47109/ Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports Kovac, Milomir Litvin, Yaroslav A. Aliev, Ruslan O. Zakirova, Elena Yu Rutland, Catrin S. Kiyasov, Andrey Rizvanov, Albert A. In this clinical study, for the first time we used the direct gene therapy to restore severe injuries of the suspensory ligament branch and superficial digital flexor tendon in horses (Equus caballus). We injected the plasmid DNA encoding two therapeutic species-specific growth factors: vascular endothelial growth factor 164 and fibroblast growth factor 2 at the site of injury in the suspensory ligament branch and tendon. Treatment effects were evaluated with the use of clinical observation and ultrasound imaging during a period of a few months. We showed that gene therapy used within a period of 2–3 months after the injury resulted in the complete recovery of functions and full restoration of the severely damaged suspensory ligament and superficial digital flexor tendon. Frontiers 2017-10-10 Article PeerReviewed Kovac, Milomir, Litvin, Yaroslav A., Aliev, Ruslan O., Zakirova, Elena Yu, Rutland, Catrin S., Kiyasov, Andrey and Rizvanov, Albert A. (2017) Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports. Frontiers in Veterinary Science, 4 (168). pp. 1-6. ISSN 2297-1769 Gene therapy Tendon Suspensory ligament Horse Vascular endothelial growth factor Fibroblast growth factor https://www.frontiersin.org/articles/10.3389/fvets.2017.00168/full doi:10.3389/fvets.2017.00168 doi:10.3389/fvets.2017.00168
spellingShingle Gene therapy
Tendon
Suspensory ligament
Horse
Vascular endothelial growth factor
Fibroblast growth factor
Kovac, Milomir
Litvin, Yaroslav A.
Aliev, Ruslan O.
Zakirova, Elena Yu
Rutland, Catrin S.
Kiyasov, Andrey
Rizvanov, Albert A.
Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title_full Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title_fullStr Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title_full_unstemmed Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title_short Gene therapy using plasmid DNA encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
title_sort gene therapy using plasmid dna encoding vascular endothelial growth factor 164 and fibroblast growth factor 2 genes for the treatment of horse tendinitis and desmitis: case reports
topic Gene therapy
Tendon
Suspensory ligament
Horse
Vascular endothelial growth factor
Fibroblast growth factor
url https://eprints.nottingham.ac.uk/47109/
https://eprints.nottingham.ac.uk/47109/
https://eprints.nottingham.ac.uk/47109/