A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering
The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a n...
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Taylor & Francis
2017
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| Online Access: | https://eprints.nottingham.ac.uk/47107/ |
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| author | Boukari, Yamina Qutachi, Omar Morris, Andrew P. Doughty, Stephen W. Billa, Nashiru |
| author_facet | Boukari, Yamina Qutachi, Omar Morris, Andrew P. Doughty, Stephen W. Billa, Nashiru |
| author_sort | Boukari, Yamina |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37°C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p < 0.05). The formulation also sintered at 37°C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37°C in situ. |
| first_indexed | 2025-11-14T20:04:22Z |
| format | Article |
| id | nottingham-47107 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:04:22Z |
| publishDate | 2017 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-471072020-05-04T19:01:12Z https://eprints.nottingham.ac.uk/47107/ A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering Boukari, Yamina Qutachi, Omar Morris, Andrew P. Doughty, Stephen W. Billa, Nashiru The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (DL-lactic-co-glycolic acid), (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37°C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively (p < 0.05). The formulation also sintered at 37°C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37°C in situ. Taylor & Francis 2017-08-17 Article PeerReviewed Boukari, Yamina, Qutachi, Omar, Morris, Andrew P., Doughty, Stephen W. and Billa, Nashiru (2017) A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering. Journal of Biomaterials Science, Polymer Edition . ISSN 0920-5063 polymeric biomaterials controlled delivery poly (lactic-co-glycolic acid) (PLGA) microspheres protein delivery tissue engineering mechanical properties formulation http://www.tandfonline.com/doi/full/10.1080/09205063.2017.1364100 doi:10.1080/09205063.2017.1364100 doi:10.1080/09205063.2017.1364100 |
| spellingShingle | polymeric biomaterials controlled delivery poly (lactic-co-glycolic acid) (PLGA) microspheres protein delivery tissue engineering mechanical properties formulation Boukari, Yamina Qutachi, Omar Morris, Andrew P. Doughty, Stephen W. Billa, Nashiru A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title | A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title_full | A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title_fullStr | A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title_full_unstemmed | A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title_short | A dual-application poly (DL-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering |
| title_sort | dual-application poly (dl-lactic-co-glycolic) acid (plga)-chitosan composite scaffold for potential use in bone tissue engineering |
| topic | polymeric biomaterials controlled delivery poly (lactic-co-glycolic acid) (PLGA) microspheres protein delivery tissue engineering mechanical properties formulation |
| url | https://eprints.nottingham.ac.uk/47107/ https://eprints.nottingham.ac.uk/47107/ https://eprints.nottingham.ac.uk/47107/ |