Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis
A new mild and efficient process for the preparation of aldehydes in water employed as flavour and fragrance components in food, beverage, cosmetics, as well as in pharmaceuticals was developed using a continuous-flow approach based on an immobilized pure transaminase-packed bed reactor. HEWT, an ω-...
| Main Authors: | , , , , |
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| Format: | Article |
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Wiley
2017
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| Online Access: | https://eprints.nottingham.ac.uk/47020/ |
| _version_ | 1848797450843717632 |
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| author | Contente, Martina L. Dall'Oglio, Federica Tamborini, Lucia Molinari, Francesco Paradisi, Francesca |
| author_facet | Contente, Martina L. Dall'Oglio, Federica Tamborini, Lucia Molinari, Francesco Paradisi, Francesca |
| author_sort | Contente, Martina L. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A new mild and efficient process for the preparation of aldehydes in water employed as flavour and fragrance components in food, beverage, cosmetics, as well as in pharmaceuticals was developed using a continuous-flow approach based on an immobilized pure transaminase-packed bed reactor. HEWT, an ω-transaminase from the haloadapted bacterium Halomonas elongata, has been selected for its excellent stability and substrate scope. Sixteen different amines were rapidly (3-15 min) oxidized into the corresponding aldehydes (90 to 99 %) with only 1 to 5 equivalents of sodium pyruvate. The process was fully automated allowing for the in-line recovery of the pure aldehydes (chemical purity >99% and isolated yields above 80%), without any further work-up procedure. |
| first_indexed | 2025-11-14T20:04:05Z |
| format | Article |
| id | nottingham-47020 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:04:05Z |
| publishDate | 2017 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-470202020-05-04T19:05:58Z https://eprints.nottingham.ac.uk/47020/ Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis Contente, Martina L. Dall'Oglio, Federica Tamborini, Lucia Molinari, Francesco Paradisi, Francesca A new mild and efficient process for the preparation of aldehydes in water employed as flavour and fragrance components in food, beverage, cosmetics, as well as in pharmaceuticals was developed using a continuous-flow approach based on an immobilized pure transaminase-packed bed reactor. HEWT, an ω-transaminase from the haloadapted bacterium Halomonas elongata, has been selected for its excellent stability and substrate scope. Sixteen different amines were rapidly (3-15 min) oxidized into the corresponding aldehydes (90 to 99 %) with only 1 to 5 equivalents of sodium pyruvate. The process was fully automated allowing for the in-line recovery of the pure aldehydes (chemical purity >99% and isolated yields above 80%), without any further work-up procedure. Wiley 2017-09-13 Article PeerReviewed Contente, Martina L., Dall'Oglio, Federica, Tamborini, Lucia, Molinari, Francesco and Paradisi, Francesca (2017) Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis. Chemcatchem . ISSN 1867-3899 http://onlinelibrary.wiley.com/doi/10.1002/cctc.201701147/abstract doi:10.1002/cctc.201701147 doi:10.1002/cctc.201701147 |
| spellingShingle | Contente, Martina L. Dall'Oglio, Federica Tamborini, Lucia Molinari, Francesco Paradisi, Francesca Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title | Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title_full | Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title_fullStr | Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title_full_unstemmed | Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title_short | Highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| title_sort | highly efficient oxidation of amines to aldehydes via flow-based biocatalysis |
| url | https://eprints.nottingham.ac.uk/47020/ https://eprints.nottingham.ac.uk/47020/ https://eprints.nottingham.ac.uk/47020/ |