Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein

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Carbene footprinting reveals binding interfaces of a multimeric membrane spanning protein

Mapping the interaction sites between membrane spanning proteins is a key challenge in structural biology. In this study a carbene footprinting approach is developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine-b...

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Bibliographic Details
Main Authors:	Manzi, Lucio, Barrow, Andrew, Hopper, Jonathan, Kaminska, Renata, Kleanthous, Colin, Robinson, Carol, Moses, John, Oldham, Neil
Format:	Article
Published:	Wiley-VCH Verlag 2017
Subjects:	carbene footprinting; integral membrane proteins; mass spectrometry; protein labelling; protein structures
Online Access:	https://eprints.nottingham.ac.uk/46932/

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