Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor
In this work, a versatile electrochemical biosensing platform was developed based on graphene/zinc oxide (G/ZnO) nanocomposite. For the synthesis of G/ZnO nanocomposite, two facile and green approaches were employed to eradicate the issues associated with conventional methods, which use harsh chemic...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2018
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| Online Access: | https://eprints.nottingham.ac.uk/46795/ |
| _version_ | 1848797402017824768 |
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| author | Low, Sze Shin |
| author_facet | Low, Sze Shin |
| author_sort | Low, Sze Shin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In this work, a versatile electrochemical biosensing platform was developed based on graphene/zinc oxide (G/ZnO) nanocomposite. For the synthesis of G/ZnO nanocomposite, two facile and green approaches were employed to eradicate the issues associated with conventional methods, which use harsh chemicals and high temperature. The G/ZnO nanocomposite synthesised via low temperature hydrothermal growth method exhibited approximate 58 times improvement in terms of sensitivity as compared to the G/ZnO nanocomposite synthesised via the mechanical stirring method. Therefore, the G/ZnO nanocomposite with higher sensitivity was employed for the following work.
Results from cyclic voltammetry and amperometry showed that the G/ZnO-modified electrode displayed a wide linear range up to 15 mM for the detection of H2O2 and sensitivity improvements of 200% in comparison to the control sample. Subsequently, an electrochemical genosensor based on G/ZnO nanocomposite was fabricated for the detection of Avian Influenza H5N1 virus. The G/ZnO-based genosensor displayed its potential in replacing the conventional detection method, with result showing higher sensitivity and efficiency. The G/ZnO-based genosensor was further applied for the detection of Coconut Cadang-Cadang Viroid disease (CCCVd) single stranded RNA (ssRNA). Promising results were obtained with high specificity in discriminating the target from mismatched ssRNA sequences. |
| first_indexed | 2025-11-14T20:03:18Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-46795 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T20:03:18Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-467952025-02-28T11:59:04Z https://eprints.nottingham.ac.uk/46795/ Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor Low, Sze Shin In this work, a versatile electrochemical biosensing platform was developed based on graphene/zinc oxide (G/ZnO) nanocomposite. For the synthesis of G/ZnO nanocomposite, two facile and green approaches were employed to eradicate the issues associated with conventional methods, which use harsh chemicals and high temperature. The G/ZnO nanocomposite synthesised via low temperature hydrothermal growth method exhibited approximate 58 times improvement in terms of sensitivity as compared to the G/ZnO nanocomposite synthesised via the mechanical stirring method. Therefore, the G/ZnO nanocomposite with higher sensitivity was employed for the following work. Results from cyclic voltammetry and amperometry showed that the G/ZnO-modified electrode displayed a wide linear range up to 15 mM for the detection of H2O2 and sensitivity improvements of 200% in comparison to the control sample. Subsequently, an electrochemical genosensor based on G/ZnO nanocomposite was fabricated for the detection of Avian Influenza H5N1 virus. The G/ZnO-based genosensor displayed its potential in replacing the conventional detection method, with result showing higher sensitivity and efficiency. The G/ZnO-based genosensor was further applied for the detection of Coconut Cadang-Cadang Viroid disease (CCCVd) single stranded RNA (ssRNA). Promising results were obtained with high specificity in discriminating the target from mismatched ssRNA sequences. 2018-02-24 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/46795/1/PhD%20Thesis%20%28edited%29%20-%20Low%20Sze%20Shin.pdf Low, Sze Shin (2018) Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor. PhD thesis, University of Nottingham. biosensors nanocomposites graphene nanotubes |
| spellingShingle | biosensors nanocomposites graphene nanotubes Low, Sze Shin Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title | Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title_full | Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title_fullStr | Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title_full_unstemmed | Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title_short | Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| title_sort | graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor |
| topic | biosensors nanocomposites graphene nanotubes |
| url | https://eprints.nottingham.ac.uk/46795/ |