Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease
Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of smoking without COPD. All COPD patients' ser...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/46770/ |
| _version_ | 1848797396551598080 |
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| author | Shindi, Reham Al-Mehairi, Amna Negm, Ola H. Kalsheker, Noor Gale, Nichola Shale, Dennis J. Harrison, Timothy W. Bolton, Charlotte E. John, Michelle Todd, Ian Tighe, Patrick J. Fairclough, Lucy C. |
| author_facet | Shindi, Reham Al-Mehairi, Amna Negm, Ola H. Kalsheker, Noor Gale, Nichola Shale, Dennis J. Harrison, Timothy W. Bolton, Charlotte E. John, Michelle Todd, Ian Tighe, Patrick J. Fairclough, Lucy C. |
| author_sort | Shindi, Reham |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of smoking without COPD. All COPD patients' sera contained elevated levels of AAbs to some of 30 autoantigens. There were significant differences in the autoantigenic specificities of IgM AAbs compared to IgG AAbs in the COPD sera: for example, AAbs to histone and scl-70 were mainly IgG, whereas AAbs to CENP-B and La/ssB were mainly IgM; by contrast, IgM and IgG AAbs to collagen-V were equally prevalent. Thus, a combination of IgM and IgG AAbs specific for multiple autoantigens are detected in all cases of COPD at a level at which all non-COPD controls are negative for AAbs. This highlights the importance of different classes of AAbs to a range of autoantigens in COPD. |
| first_indexed | 2025-11-14T20:03:13Z |
| format | Article |
| id | nottingham-46770 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:03:13Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-467702020-05-04T19:55:24Z https://eprints.nottingham.ac.uk/46770/ Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease Shindi, Reham Al-Mehairi, Amna Negm, Ola H. Kalsheker, Noor Gale, Nichola Shale, Dennis J. Harrison, Timothy W. Bolton, Charlotte E. John, Michelle Todd, Ian Tighe, Patrick J. Fairclough, Lucy C. Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of smoking without COPD. All COPD patients' sera contained elevated levels of AAbs to some of 30 autoantigens. There were significant differences in the autoantigenic specificities of IgM AAbs compared to IgG AAbs in the COPD sera: for example, AAbs to histone and scl-70 were mainly IgG, whereas AAbs to CENP-B and La/ssB were mainly IgM; by contrast, IgM and IgG AAbs to collagen-V were equally prevalent. Thus, a combination of IgM and IgG AAbs specific for multiple autoantigens are detected in all cases of COPD at a level at which all non-COPD controls are negative for AAbs. This highlights the importance of different classes of AAbs to a range of autoantigens in COPD. Elsevier 2017-10 Article PeerReviewed Shindi, Reham, Al-Mehairi, Amna, Negm, Ola H., Kalsheker, Noor, Gale, Nichola, Shale, Dennis J., Harrison, Timothy W., Bolton, Charlotte E., John, Michelle, Todd, Ian, Tighe, Patrick J. and Fairclough, Lucy C. (2017) Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease. Clinical Immunology, 183 . pp. 344-353. ISSN 1521-7035 Chronic obstructive pulmonary disease; Autoimmunity; Autoantibodies; Autoantigens; Protein microarray http://www.sciencedirect.com/science/article/pii/S1521661617301808 doi:10.1016/j.clim.2017.09.020 doi:10.1016/j.clim.2017.09.020 |
| spellingShingle | Chronic obstructive pulmonary disease; Autoimmunity; Autoantibodies; Autoantigens; Protein microarray Shindi, Reham Al-Mehairi, Amna Negm, Ola H. Kalsheker, Noor Gale, Nichola Shale, Dennis J. Harrison, Timothy W. Bolton, Charlotte E. John, Michelle Todd, Ian Tighe, Patrick J. Fairclough, Lucy C. Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title | Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title_full | Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title_fullStr | Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title_full_unstemmed | Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title_short | Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| title_sort | autoantibodies of igm and igg classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease |
| topic | Chronic obstructive pulmonary disease; Autoimmunity; Autoantibodies; Autoantigens; Protein microarray |
| url | https://eprints.nottingham.ac.uk/46770/ https://eprints.nottingham.ac.uk/46770/ https://eprints.nottingham.ac.uk/46770/ |