Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes
Background Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of...
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| Format: | Article |
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SpringerOpen
2014
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| Online Access: | https://eprints.nottingham.ac.uk/46612/ |
| _version_ | 1848797367261724672 |
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| author | Hdud, Ismail M. Mobasheri, Ali Loughna, Paul |
| author_facet | Hdud, Ismail M. Mobasheri, Ali Loughna, Paul |
| author_sort | Hdud, Ismail M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background
Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels.
Findings
Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected.
Conclusions
Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca2+. |
| first_indexed | 2025-11-14T20:02:45Z |
| format | Article |
| id | nottingham-46612 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:02:45Z |
| publishDate | 2014 |
| publisher | SpringerOpen |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-466122020-05-04T16:41:12Z https://eprints.nottingham.ac.uk/46612/ Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes Hdud, Ismail M. Mobasheri, Ali Loughna, Paul Background Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels. Findings Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected. Conclusions Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca2+. SpringerOpen 2014-01-29 Article PeerReviewed Hdud, Ismail M., Mobasheri, Ali and Loughna, Paul (2014) Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes. SpringerPlus, 3 . 59/1-59/3. ISSN 2193-1801 Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction https://springerplus.springeropen.com/articles/10.1186/2193-1801-3-59 doi:10.1186/2193-1801-3-59 doi:10.1186/2193-1801-3-59 |
| spellingShingle | Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction Hdud, Ismail M. Mobasheri, Ali Loughna, Paul Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title | Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title_full | Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title_fullStr | Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title_full_unstemmed | Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title_short | Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes |
| title_sort | effects of cyclic equibiaxial mechanical stretch on α-bk and trpv4 expression in equine chondrocytes |
| topic | Chondrocyte; Mechanical stretch; TRPV4; BK channel; Mechanotransduction |
| url | https://eprints.nottingham.ac.uk/46612/ https://eprints.nottingham.ac.uk/46612/ https://eprints.nottingham.ac.uk/46612/ |