Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity
Objective- This study investigated whether differences exist in atherogen-induced migratory behaviors and basal antioxidant enzyme capacity of vascular smooth muscle cells (VSMC) from human coronary (CA) and internal mammary (IMA) arteries. Methods- Migration experiments were performed using the Dun...
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| Format: | Article |
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Elsevier
2006
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| Online Access: | https://eprints.nottingham.ac.uk/466/ |
| _version_ | 1848790416340549632 |
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| author | Mahadevan, Vaikom Campbell, Malcolm McKeown, Pascal Bayraktutan, Ulvi |
| author_facet | Mahadevan, Vaikom Campbell, Malcolm McKeown, Pascal Bayraktutan, Ulvi |
| author_sort | Mahadevan, Vaikom |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Objective- This study investigated whether differences exist in atherogen-induced migratory behaviors and basal antioxidant enzyme capacity of vascular smooth muscle cells (VSMC)
from human coronary (CA) and internal mammary (IMA) arteries.
Methods- Migration experiments were performed using the Dunn chemotaxis chamber. The prooxidant [NAD(P)H oxidase] and antioxidant [NOS, superoxide dismutase, catalase and
glutathione peroxidase] enzyme activities were determined by specific assays.
Results- Chemotaxis experiments revealed that while both sets of VSMC migrated towards
platelet-derived growth factor-BB (1-50 ng/ml) and angiotensin II (1-50 nM), neither
oxidized-LDL (ox-LDL, 25-100 ïÂ�Âg/ml) nor native LDL (100 ïÂ�Âg/ml) affected chemotaxis in
IMA VSMC. However, high dose ox-LDL produced significant chemotaxis in CA VSMC
that was inhibited by pravastatin (100 nM), mevastatin (10 nM), losartan (10 nM), enalapril
(1 ïÂ�ÂM), and MnTBAP (a free radical scavenger, 50ïÂ� ïÂ�ÂM). Microinjection experiments with
isoprenoids i.e. geranylgeranylpyrophosphate (GGPP) and farnesylpyrophosphate (FPP)
showed distinct involvement of small GTPases in atherogen-induced VSMC migration.
Significant increases in antioxidant enzyme activities and nitrite production along with
marked decreases in NAD(P)H oxidase activity and O2
.- levels were determined in IMA
versus CA VSMC.
Conclusions- Enhanced intrinsic antioxidant capacity may confer on IMA VSMC resistance
to migration against atherogenic agents. Drugs that regulate ox-LDL or angiotensin II levels
also exert antimigratory effects. |
| first_indexed | 2025-11-14T18:12:16Z |
| format | Article |
| id | nottingham-466 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:12:16Z |
| publishDate | 2006 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-4662020-05-04T20:30:06Z https://eprints.nottingham.ac.uk/466/ Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity Mahadevan, Vaikom Campbell, Malcolm McKeown, Pascal Bayraktutan, Ulvi Objective- This study investigated whether differences exist in atherogen-induced migratory behaviors and basal antioxidant enzyme capacity of vascular smooth muscle cells (VSMC) from human coronary (CA) and internal mammary (IMA) arteries. Methods- Migration experiments were performed using the Dunn chemotaxis chamber. The prooxidant [NAD(P)H oxidase] and antioxidant [NOS, superoxide dismutase, catalase and glutathione peroxidase] enzyme activities were determined by specific assays. Results- Chemotaxis experiments revealed that while both sets of VSMC migrated towards platelet-derived growth factor-BB (1-50 ng/ml) and angiotensin II (1-50 nM), neither oxidized-LDL (ox-LDL, 25-100 ïÂ�Âg/ml) nor native LDL (100 ïÂ�Âg/ml) affected chemotaxis in IMA VSMC. However, high dose ox-LDL produced significant chemotaxis in CA VSMC that was inhibited by pravastatin (100 nM), mevastatin (10 nM), losartan (10 nM), enalapril (1 ïÂ�ÂM), and MnTBAP (a free radical scavenger, 50ïÂ� ïÂ�ÂM). Microinjection experiments with isoprenoids i.e. geranylgeranylpyrophosphate (GGPP) and farnesylpyrophosphate (FPP) showed distinct involvement of small GTPases in atherogen-induced VSMC migration. Significant increases in antioxidant enzyme activities and nitrite production along with marked decreases in NAD(P)H oxidase activity and O2 .- levels were determined in IMA versus CA VSMC. Conclusions- Enhanced intrinsic antioxidant capacity may confer on IMA VSMC resistance to migration against atherogenic agents. Drugs that regulate ox-LDL or angiotensin II levels also exert antimigratory effects. Elsevier 2006 Article PeerReviewed Mahadevan, Vaikom, Campbell, Malcolm, McKeown, Pascal and Bayraktutan, Ulvi (2006) Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity. Cardiovascular Research, 72 . pp. 60-68. Atherosclerosis Arteries Oxygen radicals Nitric oxide |
| spellingShingle | Atherosclerosis Arteries Oxygen radicals Nitric oxide Mahadevan, Vaikom Campbell, Malcolm McKeown, Pascal Bayraktutan, Ulvi Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title | Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title_full | Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title_fullStr | Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title_full_unstemmed | Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title_short | Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| title_sort | internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity |
| topic | Atherosclerosis Arteries Oxygen radicals Nitric oxide |
| url | https://eprints.nottingham.ac.uk/466/ |