Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge
The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, inc...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Elsevier
2017
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| Online Access: | https://eprints.nottingham.ac.uk/46065/ |
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| author | Clementi, Nicola Criscuolo, Elena Cappelletti, Francesca Quaranta, Paola Pistello, Mauro Diotti, Roberta A. Sautto, Giuseppe A. Tarr, Alexander W. Mailland, Federico Concas, Daniela Burioni, Roberto Clementi, Massimo Mancini, Nicasio |
| author_facet | Clementi, Nicola Criscuolo, Elena Cappelletti, Francesca Quaranta, Paola Pistello, Mauro Diotti, Roberta A. Sautto, Giuseppe A. Tarr, Alexander W. Mailland, Federico Concas, Daniela Burioni, Roberto Clementi, Massimo Mancini, Nicasio |
| author_sort | Clementi, Nicola |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission. |
| first_indexed | 2025-11-14T20:00:54Z |
| format | Article |
| id | nottingham-46065 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T20:00:54Z |
| publishDate | 2017 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-460652020-05-04T19:56:31Z https://eprints.nottingham.ac.uk/46065/ Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge Clementi, Nicola Criscuolo, Elena Cappelletti, Francesca Quaranta, Paola Pistello, Mauro Diotti, Roberta A. Sautto, Giuseppe A. Tarr, Alexander W. Mailland, Federico Concas, Daniela Burioni, Roberto Clementi, Massimo Mancini, Nicasio The present study focused on inhibition of HSV-1 and -2 replication and pathogenesis in vitro and in vivo, through the selective targeting of the envelope glycoprotein D. Firstly, a human monoclonal antibody (Hu-mAb#33) was identified that could neutralise both HSV-1 and -2 at nM concentrations, including clinical isolates from patients affected by different clinical manifestations and featuring different susceptibility to acyclovir in vitro. Secondly, the potency of inhibition of both infection by cell-free viruses and cell-to-cell virus transmission was also assessed. Finally, mice receiving a single systemic injection of Hu-mAb#33 were protected from death and severe clinical manifestations following both ocular and vaginal HSV-1 and -2 lethal challenge. These results pave the way for further studies reassessing the importance of HSV entry as a novel target for therapeutic intervention and inhibition of cell-to-cell virus transmission. Elsevier 2017-07 Article PeerReviewed Clementi, Nicola, Criscuolo, Elena, Cappelletti, Francesca, Quaranta, Paola, Pistello, Mauro, Diotti, Roberta A., Sautto, Giuseppe A., Tarr, Alexander W., Mailland, Federico, Concas, Daniela, Burioni, Roberto, Clementi, Massimo and Mancini, Nicasio (2017) Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge. Antiviral Research, 143 . pp. 48-61. ISSN 1872-9096 HSV disseminated infection; Cell-to-cell virus transmission; Human antibodies; In vivo protection http://www.sciencedirect.com/science/article/pii/S0166354216307562 doi:10.1016/j.antiviral.2017.03.028 doi:10.1016/j.antiviral.2017.03.028 |
| spellingShingle | HSV disseminated infection; Cell-to-cell virus transmission; Human antibodies; In vivo protection Clementi, Nicola Criscuolo, Elena Cappelletti, Francesca Quaranta, Paola Pistello, Mauro Diotti, Roberta A. Sautto, Giuseppe A. Tarr, Alexander W. Mailland, Federico Concas, Daniela Burioni, Roberto Clementi, Massimo Mancini, Nicasio Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title | Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title_full | Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title_fullStr | Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title_full_unstemmed | Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title_short | Entry inhibition of HSV-1 and -2 protects mice from viral lethal challenge |
| title_sort | entry inhibition of hsv-1 and -2 protects mice from viral lethal challenge |
| topic | HSV disseminated infection; Cell-to-cell virus transmission; Human antibodies; In vivo protection |
| url | https://eprints.nottingham.ac.uk/46065/ https://eprints.nottingham.ac.uk/46065/ https://eprints.nottingham.ac.uk/46065/ |