Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media

An aqueous process for the recovery of oil bodies from rapeseed using sodium bicarbonate-based soaking and grinding media (pH 9.5) was investigated. The effect of the ratio between seed and mass of media during grinding and molarity of the medium used on oil body integrity, purity and storage stabil...

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Main Authors: De Chirico, Simone, Di Bari, Vicenzo, Foster, Tim, Gray, David A.
Format: Article
Published: Elsevier 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/45823/
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author De Chirico, Simone
Di Bari, Vicenzo
Foster, Tim
Gray, David A.
author_facet De Chirico, Simone
Di Bari, Vicenzo
Foster, Tim
Gray, David A.
author_sort De Chirico, Simone
building Nottingham Research Data Repository
collection Online Access
description An aqueous process for the recovery of oil bodies from rapeseed using sodium bicarbonate-based soaking and grinding media (pH 9.5) was investigated. The effect of the ratio between seed and mass of media during grinding and molarity of the medium used on oil body integrity, purity and storage stability have been studied. The grinding of seeds in solution at a ratio of 1:7 (w/w) significantly improved the quality of oil body suspension to a size more in-line with that seen in vivo (average D4,3 of 1.19 μm). The purity and the composition of the recovered oil bodies depends on the molarity of medium used; the use of a sodium bicarbonate solution (pH 9.5, 0.1 M) in the grinding and washing steps produced oil body preparations with the same purity as that resulting from washing a crude preparation with 9 M urea. The resultant emulsion had improved physical stability over a storage period of one month.
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spelling nottingham-458232020-05-04T19:32:25Z https://eprints.nottingham.ac.uk/45823/ Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media De Chirico, Simone Di Bari, Vicenzo Foster, Tim Gray, David A. An aqueous process for the recovery of oil bodies from rapeseed using sodium bicarbonate-based soaking and grinding media (pH 9.5) was investigated. The effect of the ratio between seed and mass of media during grinding and molarity of the medium used on oil body integrity, purity and storage stability have been studied. The grinding of seeds in solution at a ratio of 1:7 (w/w) significantly improved the quality of oil body suspension to a size more in-line with that seen in vivo (average D4,3 of 1.19 μm). The purity and the composition of the recovered oil bodies depends on the molarity of medium used; the use of a sodium bicarbonate solution (pH 9.5, 0.1 M) in the grinding and washing steps produced oil body preparations with the same purity as that resulting from washing a crude preparation with 9 M urea. The resultant emulsion had improved physical stability over a storage period of one month. Elsevier 2018-02-15 Article PeerReviewed De Chirico, Simone, Di Bari, Vicenzo, Foster, Tim and Gray, David A. (2018) Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media. Food Chemistry, 241 . pp. 419-426. ISSN 0308-8146 Emulsions; Oil bodies; Rapeseed; Sodium bicarbonate solution; Alkaline pH; Exogenous proteins; Storage stability http://www.sciencedirect.com/science/article/pii/S0308814617314620 doi:10.1016/j.foodchem.2017.09.008 doi:10.1016/j.foodchem.2017.09.008
spellingShingle Emulsions; Oil bodies; Rapeseed; Sodium bicarbonate solution; Alkaline pH; Exogenous proteins; Storage stability
De Chirico, Simone
Di Bari, Vicenzo
Foster, Tim
Gray, David A.
Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title_full Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title_fullStr Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title_full_unstemmed Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title_short Enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
title_sort enhancing the recovery of oilseed rape seed oil bodies (oleosomes) using bicarbonate-based soaking and grinding media
topic Emulsions; Oil bodies; Rapeseed; Sodium bicarbonate solution; Alkaline pH; Exogenous proteins; Storage stability
url https://eprints.nottingham.ac.uk/45823/
https://eprints.nottingham.ac.uk/45823/
https://eprints.nottingham.ac.uk/45823/