The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers
The tumour microenvironment consists of malignant cells, stroma, and immune cells. The role of adaptive immunity in inducing a pathological complete response (pCR) in breast cancer with neoadjuvant chemotherapy (NAC) is well studied. The contribution of innate immunity, however, is poorly documented...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Hindawi
2017
|
| Online Access: | https://eprints.nottingham.ac.uk/45598/ |
| _version_ | 1848797162740121600 |
|---|---|
| author | Kaewkangsadan, Viriya Verma, Chandan Eremin, Jennifer M. Cowley, Gerard P. Ilyas, Mohammad Satthaporn, Sukchai Eremin, Oleg |
| author_facet | Kaewkangsadan, Viriya Verma, Chandan Eremin, Jennifer M. Cowley, Gerard P. Ilyas, Mohammad Satthaporn, Sukchai Eremin, Oleg |
| author_sort | Kaewkangsadan, Viriya |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The tumour microenvironment consists of malignant cells, stroma, and immune cells. The role of adaptive immunity in inducing a pathological complete response (pCR) in breast cancer with neoadjuvant chemotherapy (NAC) is well studied. The contribution of innate immunity, however, is poorly documented. Breast tumours and axillary lymph nodes (ALNs) from 33 women with large and locally advanced breast cancers (LLABCs) undergoing NAC were immunohistochemically assessed for tumour-infiltrating macrophages (TIMs: M1 and M2), neutrophils (TINs), and dendritic cells (TIDCs) using labelled antibodies and semiquantitative methods. Patients’ blood neutrophils (n = 108), DCs (mDC1 and pDC), and their costimulatory molecules (n = 30) were also studied. Pathological results were classified as pCR, good (GPR) or poor (PRR). In breast and metastatic ALNs, high levels of CD163+ TIMs were significantly associated with a pCR. In blood, high levels of neutrophils were significantly associated with pCR in metastatic ALNs, whilst the % of mDC1 and pDC and expression of HLA-DR, mDC1 CD40, and CD83 were significantly reduced. NAC significantly reduced tumour DCs but increased blood DCs. PPRs to NAC had significantly reduced HLA-DR, CD40, and CD86 expression. Our study demonstrated novel findings documenting the differential but important contributions of innate immunity to pCRs in patients with LLABCs undergoing NAC. |
| first_indexed | 2025-11-14T19:59:30Z |
| format | Article |
| id | nottingham-45598 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:59:30Z |
| publishDate | 2017 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-455982020-05-04T19:02:10Z https://eprints.nottingham.ac.uk/45598/ The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers Kaewkangsadan, Viriya Verma, Chandan Eremin, Jennifer M. Cowley, Gerard P. Ilyas, Mohammad Satthaporn, Sukchai Eremin, Oleg The tumour microenvironment consists of malignant cells, stroma, and immune cells. The role of adaptive immunity in inducing a pathological complete response (pCR) in breast cancer with neoadjuvant chemotherapy (NAC) is well studied. The contribution of innate immunity, however, is poorly documented. Breast tumours and axillary lymph nodes (ALNs) from 33 women with large and locally advanced breast cancers (LLABCs) undergoing NAC were immunohistochemically assessed for tumour-infiltrating macrophages (TIMs: M1 and M2), neutrophils (TINs), and dendritic cells (TIDCs) using labelled antibodies and semiquantitative methods. Patients’ blood neutrophils (n = 108), DCs (mDC1 and pDC), and their costimulatory molecules (n = 30) were also studied. Pathological results were classified as pCR, good (GPR) or poor (PRR). In breast and metastatic ALNs, high levels of CD163+ TIMs were significantly associated with a pCR. In blood, high levels of neutrophils were significantly associated with pCR in metastatic ALNs, whilst the % of mDC1 and pDC and expression of HLA-DR, mDC1 CD40, and CD83 were significantly reduced. NAC significantly reduced tumour DCs but increased blood DCs. PPRs to NAC had significantly reduced HLA-DR, CD40, and CD86 expression. Our study demonstrated novel findings documenting the differential but important contributions of innate immunity to pCRs in patients with LLABCs undergoing NAC. Hindawi 2017-08-23 Article PeerReviewed Kaewkangsadan, Viriya, Verma, Chandan, Eremin, Jennifer M., Cowley, Gerard P., Ilyas, Mohammad, Satthaporn, Sukchai and Eremin, Oleg (2017) The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers. Journal of Immunology Research, 2017 . 1049023/1-1049023/21. ISSN 2314-7156 https://doi.org/10.1155/2017/1049023 doi:10.1155/2017/1049023 doi:10.1155/2017/1049023 |
| spellingShingle | Kaewkangsadan, Viriya Verma, Chandan Eremin, Jennifer M. Cowley, Gerard P. Ilyas, Mohammad Satthaporn, Sukchai Eremin, Oleg The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title | The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title_full | The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title_fullStr | The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title_full_unstemmed | The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title_short | The differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| title_sort | differential contribution of the innate immune system to a good pathological response in the breast and axillary lymph nodes induced by neoadjuvant chemotherapy in women with large and locally advanced breast cancers |
| url | https://eprints.nottingham.ac.uk/45598/ https://eprints.nottingham.ac.uk/45598/ https://eprints.nottingham.ac.uk/45598/ |