Development and validation of a high-density SNP genotyping array for African Oil Palm
High-density single nucleotide polymorphism (SNP) genotyping arrays are powerful tools that can measure the level of genetic polymorphism within a population. To develop a whole-genome SNP array for oil palms, SNP discovery was performed using deep resequencing of eight libraries derived from 132 El...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
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Cell Press/Elsevier
2016
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| Online Access: | https://eprints.nottingham.ac.uk/45372/ |
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| author | Kwong, Qi Bin Teh, Chee Keng Ong, Ai Ling Heng, Huey Ying Lee, Heng Leng Mohamed, Mohaimi Low, Joel Zi-Bin Apparow, Sukganah Chew, Fook Tim Mayes, Sean Kulaveerasingam, Harikrishna Tammi, Martti Appleton, David Ross |
| author_facet | Kwong, Qi Bin Teh, Chee Keng Ong, Ai Ling Heng, Huey Ying Lee, Heng Leng Mohamed, Mohaimi Low, Joel Zi-Bin Apparow, Sukganah Chew, Fook Tim Mayes, Sean Kulaveerasingam, Harikrishna Tammi, Martti Appleton, David Ross |
| author_sort | Kwong, Qi Bin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | High-density single nucleotide polymorphism (SNP) genotyping arrays are powerful tools that can measure the level of genetic polymorphism within a population. To develop a whole-genome SNP array for oil palms, SNP discovery was performed using deep resequencing of eight libraries derived from 132 Elaeis guineensis and Elaeis oleifera palms belonging to 59 origins, resulting in the discovery of >3 million putative SNPs. After SNP filtering, the Illumina OP200K custom array was built with 170 860 successful probes. Phenetic clustering analysis revealed that the array could distinguish between palms of different origins in a way consistent with pedigree records. Genome-wide linkage disequilibrium declined more slowly for the commercial populations (ranging from 120 kb at r2 = 0.43 to 146 kb at r2 = 0.50) when compared with the semi-wild populations (19.5 kb at r2 = 0.22). Genetic fixation mapping comparing the semi-wild and commercial population identified 321 selective sweeps. A genome-wide association study (GWAS) detected a significant peak on chromosome 2 associated with the polygenic component of the shell thickness trait (based on the trait shell-to-fruit; S/F %) in tenera palms. Testing of a genomic selection model on the same trait resulted in good prediction accuracy (r = 0.65) with 42% of the S/F%variation explained. The first high-density SNP genotyping array for oil palm has been developed and shown to be robust for use in genetic studies and with potential for developing early trait prediction to shorten the oil palm breeding cycle. |
| first_indexed | 2025-11-14T19:58:48Z |
| format | Article |
| id | nottingham-45372 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:58:48Z |
| publishDate | 2016 |
| publisher | Cell Press/Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-453722020-05-04T17:58:30Z https://eprints.nottingham.ac.uk/45372/ Development and validation of a high-density SNP genotyping array for African Oil Palm Kwong, Qi Bin Teh, Chee Keng Ong, Ai Ling Heng, Huey Ying Lee, Heng Leng Mohamed, Mohaimi Low, Joel Zi-Bin Apparow, Sukganah Chew, Fook Tim Mayes, Sean Kulaveerasingam, Harikrishna Tammi, Martti Appleton, David Ross High-density single nucleotide polymorphism (SNP) genotyping arrays are powerful tools that can measure the level of genetic polymorphism within a population. To develop a whole-genome SNP array for oil palms, SNP discovery was performed using deep resequencing of eight libraries derived from 132 Elaeis guineensis and Elaeis oleifera palms belonging to 59 origins, resulting in the discovery of >3 million putative SNPs. After SNP filtering, the Illumina OP200K custom array was built with 170 860 successful probes. Phenetic clustering analysis revealed that the array could distinguish between palms of different origins in a way consistent with pedigree records. Genome-wide linkage disequilibrium declined more slowly for the commercial populations (ranging from 120 kb at r2 = 0.43 to 146 kb at r2 = 0.50) when compared with the semi-wild populations (19.5 kb at r2 = 0.22). Genetic fixation mapping comparing the semi-wild and commercial population identified 321 selective sweeps. A genome-wide association study (GWAS) detected a significant peak on chromosome 2 associated with the polygenic component of the shell thickness trait (based on the trait shell-to-fruit; S/F %) in tenera palms. Testing of a genomic selection model on the same trait resulted in good prediction accuracy (r = 0.65) with 42% of the S/F%variation explained. The first high-density SNP genotyping array for oil palm has been developed and shown to be robust for use in genetic studies and with potential for developing early trait prediction to shorten the oil palm breeding cycle. Cell Press/Elsevier 2016-08-01 Article PeerReviewed Kwong, Qi Bin, Teh, Chee Keng, Ong, Ai Ling, Heng, Huey Ying, Lee, Heng Leng, Mohamed, Mohaimi, Low, Joel Zi-Bin, Apparow, Sukganah, Chew, Fook Tim, Mayes, Sean, Kulaveerasingam, Harikrishna, Tammi, Martti and Appleton, David Ross (2016) Development and validation of a high-density SNP genotyping array for African Oil Palm. Molecular Plant, 9 (8). pp. 1132-1141. ISSN 1752-9867 oil palm whole-genome SNP array genome-wide association study genomic selection genetic fixation genome-wide linkage disequilibrium http://www.sciencedirect.com/science/article/pii/S1674205216300326?via%3Dihub doi:10.1016/j.molp.2016.04.010 doi:10.1016/j.molp.2016.04.010 |
| spellingShingle | oil palm whole-genome SNP array genome-wide association study genomic selection genetic fixation genome-wide linkage disequilibrium Kwong, Qi Bin Teh, Chee Keng Ong, Ai Ling Heng, Huey Ying Lee, Heng Leng Mohamed, Mohaimi Low, Joel Zi-Bin Apparow, Sukganah Chew, Fook Tim Mayes, Sean Kulaveerasingam, Harikrishna Tammi, Martti Appleton, David Ross Development and validation of a high-density SNP genotyping array for African Oil Palm |
| title | Development and validation of a high-density
SNP genotyping array for African Oil Palm |
| title_full | Development and validation of a high-density
SNP genotyping array for African Oil Palm |
| title_fullStr | Development and validation of a high-density
SNP genotyping array for African Oil Palm |
| title_full_unstemmed | Development and validation of a high-density
SNP genotyping array for African Oil Palm |
| title_short | Development and validation of a high-density
SNP genotyping array for African Oil Palm |
| title_sort | development and validation of a high-density
snp genotyping array for african oil palm |
| topic | oil palm whole-genome SNP array genome-wide association study genomic selection genetic fixation genome-wide linkage disequilibrium |
| url | https://eprints.nottingham.ac.uk/45372/ https://eprints.nottingham.ac.uk/45372/ https://eprints.nottingham.ac.uk/45372/ |