The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments)
Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendatio...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2017
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| Online Access: | https://eprints.nottingham.ac.uk/45121/ |
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| author | Percie du Sert, Nathalie Alfieri, Alessio Allan, Stuart M. Carswell, Hilary V.O. Deuchar, Graeme A. Farr, Tracy D. Flecknell, Paul Gallagher, Lindsay Gibson, Claire L. Haley, Michael J. Macleod, Malcolm R. McColl, Barry W. McCabe, Christopher Morancho, Anna Moon, Lawrence D.F. O’Neill, Michael J. Pérez de Puig, Isabel Planas, Anna Ragan, C. Ian Rosell, Anna Roy, Lisa A. Ryder, Kathryn O. Simats, Alba Sena, Emily S. Sutherland, Brad A. Tricklebank, Mark D. Trueman, Rebecca C. Whitfield, Lucy Wong, Raymond Macrae, I. Mhairi |
| author_facet | Percie du Sert, Nathalie Alfieri, Alessio Allan, Stuart M. Carswell, Hilary V.O. Deuchar, Graeme A. Farr, Tracy D. Flecknell, Paul Gallagher, Lindsay Gibson, Claire L. Haley, Michael J. Macleod, Malcolm R. McColl, Barry W. McCabe, Christopher Morancho, Anna Moon, Lawrence D.F. O’Neill, Michael J. Pérez de Puig, Isabel Planas, Anna Ragan, C. Ian Rosell, Anna Roy, Lisa A. Ryder, Kathryn O. Simats, Alba Sena, Emily S. Sutherland, Brad A. Tricklebank, Mark D. Trueman, Rebecca C. Whitfield, Lucy Wong, Raymond Macrae, I. Mhairi |
| author_sort | Percie du Sert, Nathalie |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information). |
| first_indexed | 2025-11-14T19:58:05Z |
| format | Article |
| id | nottingham-45121 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:58:05Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-451212020-05-04T19:00:11Z https://eprints.nottingham.ac.uk/45121/ The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) Percie du Sert, Nathalie Alfieri, Alessio Allan, Stuart M. Carswell, Hilary V.O. Deuchar, Graeme A. Farr, Tracy D. Flecknell, Paul Gallagher, Lindsay Gibson, Claire L. Haley, Michael J. Macleod, Malcolm R. McColl, Barry W. McCabe, Christopher Morancho, Anna Moon, Lawrence D.F. O’Neill, Michael J. Pérez de Puig, Isabel Planas, Anna Ragan, C. Ian Rosell, Anna Roy, Lisa A. Ryder, Kathryn O. Simats, Alba Sena, Emily S. Sutherland, Brad A. Tricklebank, Mark D. Trueman, Rebecca C. Whitfield, Lucy Wong, Raymond Macrae, I. Mhairi Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information). Nature Publishing Group 2017-08-11 Article PeerReviewed Percie du Sert, Nathalie, Alfieri, Alessio, Allan, Stuart M., Carswell, Hilary V.O., Deuchar, Graeme A., Farr, Tracy D., Flecknell, Paul, Gallagher, Lindsay, Gibson, Claire L., Haley, Michael J., Macleod, Malcolm R., McColl, Barry W., McCabe, Christopher, Morancho, Anna, Moon, Lawrence D.F., O’Neill, Michael J., Pérez de Puig, Isabel, Planas, Anna, Ragan, C. Ian, Rosell, Anna, Roy, Lisa A., Ryder, Kathryn O., Simats, Alba, Sena, Emily S., Sutherland, Brad A., Tricklebank, Mark D., Trueman, Rebecca C., Whitfield, Lucy, Wong, Raymond and Macrae, I. Mhairi (2017) The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments). Journal of Cerebral Blood Flow & Metabolism . ISSN 1559-7016 3Rs Animal welfare Guidelines Middle cerebral artery occlusion Stroke http://journals.sagepub.com/doi/10.1177/0271678X17709185 doi:10.1177/0271678X17709185 doi:10.1177/0271678X17709185 |
| spellingShingle | 3Rs Animal welfare Guidelines Middle cerebral artery occlusion Stroke Percie du Sert, Nathalie Alfieri, Alessio Allan, Stuart M. Carswell, Hilary V.O. Deuchar, Graeme A. Farr, Tracy D. Flecknell, Paul Gallagher, Lindsay Gibson, Claire L. Haley, Michael J. Macleod, Malcolm R. McColl, Barry W. McCabe, Christopher Morancho, Anna Moon, Lawrence D.F. O’Neill, Michael J. Pérez de Puig, Isabel Planas, Anna Ragan, C. Ian Rosell, Anna Roy, Lisa A. Ryder, Kathryn O. Simats, Alba Sena, Emily S. Sutherland, Brad A. Tricklebank, Mark D. Trueman, Rebecca C. Whitfield, Lucy Wong, Raymond Macrae, I. Mhairi The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title | The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title_full | The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title_fullStr | The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title_full_unstemmed | The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title_short | The IMPROVE guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments) |
| title_sort | improve guidelines (ischaemia models: procedural refinements of in vivo experiments) |
| topic | 3Rs Animal welfare Guidelines Middle cerebral artery occlusion Stroke |
| url | https://eprints.nottingham.ac.uk/45121/ https://eprints.nottingham.ac.uk/45121/ https://eprints.nottingham.ac.uk/45121/ |